白血病异基因造血干细胞移植后TCR Vβ亚家族基因谱的研究  

Study on TCR Vβ subfamily genes following allogeneic hematopoietic stem cell transplantation in leukemic patients

作　　者：杜 欣[1] 李扬秋[2] 李树浓[3] 

机构地区：[1]广东省人民医院血液科,广东广州510080 [2]暨南大学血液病研究室,广东广州510087 [3]中山医科大学病理生理教研室,广东广州510082

出　　处：《免疫学杂志》2002年第3期211-217,共7页Immunological Journal

基　　金：广东省1998年重点攻关基金资助项目(1998017)

摘　　要：目的 了解白血病病人异基因外周血干细胞移植后1年以上患者TCR VβT细胞免疫功能重建的情况。方法 采用 RT-PCR扩增5例CML和1例AML-M3a移植后（12-56个月）患者外周血的单个核细胞的 TCR Vβ 24个亚家族基因,分析其TCR Vβ亚家族的表达。结果 经24个 Vβ引物所分别进行的RT-PCR检测TCR Vβ各亚家族基因的表达情况,发现6例病人外周血与正常人表达24个Vβ亚家族有明显的不同,病人的部分Vβ亚家族T细胞仍未能重建,仅表达5-22个亚家族基因。结论 移植后1年以上患者尽管CD3+T细胞已恢复,但外周血TCR Vβ亚家族基因谱的表达仍不完全,T细胞免疫功能仍未完全重建。ve To investigate the reconstitution of T cell receptor repertoire one year after allogeneic hematopoietic stem cell transplantation. Methods TCR Vβ subfamily genes in peripheral blood mononuclear cells from 5 cases of chronic leukemia and a case of acute leukemia were amplified using RT-PCR. Results There were marked difference between these patients and normal controls. Only 5 -22Vβ subfamily T cells were found in samples from these patients, and there were different patterns in different patients. Conclusion TCR repertoire complexity was abnormal in all patients after one year after transplantation of allogeneic hemopoitic stem cells, although the number of CD3+ T cell recovered.

关 键 词：TCRVΒ基因 免疫重建 白血病 外周血干细胞移植 RT-PCR 

分 类 号：R457.7[医药卫生—治疗学]