夹心免疫PCR方法检测蓖麻毒素  

Establishment of sandwich immuno-PCR technique for detection of ricin

作　　者：宋云扬[1] 刘 娟[1] 应天翼[1] 郝兰群[1] 张 靖[1] 李艳军[1] 王惠芳[1] 

机构地区：[1]北京药物化学研究所生化室,北京102205

出　　处：《免疫学杂志》2002年第3期229-231,共3页Immunological Journal

摘　　要：目的 建立了双抗夹心免疫PCR的检测技术,检测极微量的抗原。方法 以亲和素作为连接分子连接生物素化抗体和生物素化DNA,应用于免疫PCR中,检测极微量的抗原。结果 建立了双抗夹心兔疫PCR的检测技术,检测蓖麻毒素的灵敏度达0.1pg/mL,与夹心ELISA方法比较灵敏度高103。结论 建立的免疫PCR系统灵敏度高,可用于各种微量抗原的检测。ve To develops sandwich immune-PCR system for monitoring antigen. Methods Avidin was used to bridge the biotinylated antibodies and biotinylated DNA, and detect antigen by immuno-PCR. Results As little as 0.1 pg/mL of ricin could be detected by the sandwich immuno-PCR, which was 103 times more sensitive than parallel ELISA control. Conclusion The universal immuno-PCR sys-tem can be used to detect ultra-micro antigen.

关 键 词：蓖麻毒素 免疫PCR 戊二醛 生物素 亲和素 中药 

分 类 号：R392-33[医药卫生—免疫学]