不同发育阶段人皮肤表皮干细胞增殖分化特征及其与创面修复结局关系的研究  

THE CHARACTERISTICS OF STEM CELL DIFFERENTIATION IN HUMAN SKINS FROM DIFFERENT DEVELOPED STAGES AND THEIR EFFECTS ON SKIN WOUND REPAIR

作　　者：赵志力[1] 付小兵[1] 孙同柱[1] 杨银辉[1] 陈伟[1] 孙晓庆[1] 盛志勇[1] 

机构地区：[1]解放军第304医院

出　　处：《解放军医学杂志》2002年第5期388-389,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家重大基础研究规划项目 (编号G1 9990 542 0 4 );国家自然科学基金(编号 30 1 70 966);国家杰出青年科学基金 (编号 3952 50 2 4 )

摘　　要：为从细胞分化角度研究人胎儿期、少儿期、成年人期皮肤中表皮干细胞增殖分化特征 ,以及这些特征与创面修复结局的关系 ,分别取因创伤等原因致流产的 2 2～ 2 4周龄胎儿和 4～ 12周岁少儿、35～ 5 3岁成年人 3组全层皮肤。采用免疫组化方法检测表皮干细胞特异表达的β1整合素和细胞角蛋白 19(K19)。结果发现 ,胎儿期皮肤表皮基底层细胞β1整合素和K19染色均为强阳性 ,少儿期表皮基底层细胞中 6 0 %～80 %的细胞表达β1整合素和K19,成年人表皮基底层中表达β1整合素和K19的细胞较少儿组进一步减少 ,且染色强度较弱。结果提示 ,胎儿期表皮基底层增殖细胞均为表皮干细胞和短暂扩充细胞 ,而少儿期表皮基底层中部分细胞为干细胞和短暂扩充细胞 ,成年人期干细胞与短暂扩充细胞所占的比例则进一步降低。To gain insight into the mechanisms of an age related difference in ability of wound healing, the characteristics of stem cell differentiation in skins from fetus, child and adult were investiga ted. Integrin β 1 and keratin 19 (K19) were used as the biochemical markers for stem cells and transit amplifying cells. Biopsies were taken from fetus (22～24week gestational age), children (4～12year) and adults (35～53year). Immunohistochemistry was used. As for the immunostainings of fetal tissue sections, integrin β 1 and K19 expressions were observed in all epidermal basal cells. In children skin, the ratio of integrin β 1 and K19 positive cells in the epidermal basal layer was 60%～80%. In adults, the ratio in the epidermal layer decreased. These results indicate that fetal skin epidermis contains a large number of stem cells and transit amplifying cells, and the proportion of stem cells and transit amplifying cells decreases with age after birth, which maybe a reason of the age associated difference in ability of wound healing.

关 键 词：表皮干细胞 Β1整合素 角蛋白19 细胞增殖 细胞分化 创伤 烧伤 创面修复 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R644[医药卫生—基础医学]