人半月板纤维软骨细胞培养及生物学特性研究  

CULTURE OF MENISCAL CARTILAGE CELLS AND THEIR BIOLOGICAL BEHAVIORS in vitro

作　　者：祝云利[1] 荆鑫[1] 缪志和[1] 吴海山[1] 周维江[1] 

机构地区：[1]第二军医大学长征医院

出　　处：《解放军医学杂志》2002年第5期432-433,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39770 743)

摘　　要：为探索分离培养人半月板纤维软骨细胞的简便实用方法 ,采用胰蛋白酶和胶原酶联合消化的方法 ,简便快速地获得大量成活率高的人半月板纤维软骨细胞。在F 12培养液中进行原代和传代培养 ,并对传代培养纤维软骨细胞进行了免疫组化鉴定 ,光镜及超微结构的观察。结果显示 ,光镜下 ,原代培养细胞呈线形 ,单层排列 ,电镜可见细胞内有丰富的粗面内质网及线粒体 ,细胞呈多极性 ,表面有突起 ;纤维软骨细胞免疫组化Ⅱ型胶原染色阳性。To investigate the behavior of meniscal fibrocartilage cells in vitro ,human meniscal fibrocartilage cells were harvested from adolescents and sequentially digestied with 0 50% trypsin and 0 2% collagenase (type2). The isolated cells were cultured in Ham'sF 12 medium and identified by histochemical methods. Cell proliferation, morphology and ultrastructure were observed by phase contrast microscope and electronic microscope. In primarily cultured cells, fusiform cells dominated and they were confirmed by the positive result of immunohistochemical examination for type collagen staining. Our results indicate that it is a simple and highly effective method.

关 键 词：半月板 纤维软骨 细胞培养 组织工程学 生物学特性 

分 类 号：R684[医药卫生—骨科学] R318.01[医药卫生—外科学]