高温高湿环境犬肢体火器伤后TNF-α与HSP70含量变化的实验研究  

STUDY ON DYNAMIC CHANGE OF TNF-α AND HSP70 AFTER GUNSHOT WOUND ON LIMBS OF CANINE IN HOT AND HUMID ENVIRONMENT

作　　者：张旭辉[1] 裴国献[1] 魏宽海[1] 周祥吉[1] 

机构地区：[1]第一军医大学南方医院

出　　处：《解放军医学杂志》2002年第5期442-444,共3页Medical Journal of Chinese People's Liberation Army

基　　金：全军"九五"医药卫生杰出中青年基金资助课题 (编号 98J0 0 4 )

摘　　要：为探讨高温高湿环境下肢体火器伤后肿瘤坏死因子 α(TNF α)与热休克蛋白 70 (HSP70 )含量变化的规律 ,将犬随机分为常温常湿组、热适应组、高温高湿组 ,分别在致伤前和火器伤后 1、3、4、6、8、10、14、18、2 4h检测TNF α与HSP70含量。结果发现 ,①伤道骨骼肌中TNF α较血浆中升高明显。常温常湿组 4h开始升高 ,14h达高峰 ;高温高湿组 1h升高 ,10h达到高峰 ,其峰值显著高于常温常湿组(P <0 0 5 ) ;热适应组变化基本和高温高湿组一致 ,只是升高幅度较低 (P<0 0 5 )。②淋巴细胞中HSP70含量在高温高湿组有两个高峰 ,分别为伤后 4h和 14～ 18h ;热适应组 3h达到高峰 ,持续高峰至 6h ;常温常湿组 6h达到高峰 ,且其伤道骨骼肌HSP70含量在伤后 14～ 18h也出现一个增幅不明显的高峰期。说明高温高湿环境下肢体火器伤后TNF α与HSP70含量增高 。To investigate the dynamic change of TNF α and HSP70 after gunshot wound on limbs of canine in hot and humid environment, the canines were divided into three groups: trauma in normal environment group(NE), trauma in heat acclimatization group (HA), trauma in hot and humid environment group(HHE). Tumor necrosis factor α (TNF α) and HSP70 were measured at the time of 0, 1, 3, 4, 6 ,8 , 10, 14, 18, 24h after canine being gunshot. The results showed: ①TNF α in muscle tissue from gunshot wound tract was significantly higher than that in blood plasma( P <0 05). TNF α was ascending at 4h in NE group, and peaked at 14h. Numerical value in HHE group was at 3h and 10h, the maximum in HHE group was significantly higher than that in NE group( P <0 05). TNF α in HA group was the same as that in HHE, the maximum in HA group was significantly lower than that in HHE group ( P <0 05). ②HSP70 in lymphocyte ascended to the peak at 4h and 14～18h respectively; there was one peak at 3h in heat acclimatization group, and lasted to 6h; HSP70 ascended to the peak at 6h in normal environment group; there was no obvious peak in muscle tissue from gunshot wound tract at 14～18h after canine being gunshot. Special changes of TNF α and HSP70 were found after canine being gunshot in hot and humid environment, which were different from those in the normal environment.

关 键 词：高温高湿环境 创伤 肿瘤坏死因子 热休克蛋白质类70 火器伤 动物实验 下肢损伤 

分 类 号：R826.68[医药卫生—临床医学]