HPLC测定不同产地北柴胡中的柴胡皂苷a、c、d  

Determination of saikosaponin a, c, d in Bupleurum Chinense DC. from different areas by HPLC

作　　者：林东昊[1] 茅仁刚[1] 王智华[2] 洪筱坤[2] 潘胜利[3] 

机构地区：[1]上海师范大学新康制药厂,上海200234 [2]上海中医药大学,上海200032 [3]复旦大学药学院,上海200032

出　　处：《中成药》2002年第5期382-384,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :建立一个RP HPLC方法 ,对国内 2 0个不同产地北柴胡样品中的柴胡皂苷a、c、d进行含量测定。方法 :以ODSC1 8色谱柱、乙腈 水为流动相进行梯度洗脱 ,检测波长为 2 1 0nm。结果 :柴胡皂苷a、c、d的线性回归方程分别为Yssa=2 .0 377× 1 0 5+1 .1 555× 1 0 6 Xssa(r =0 .9999)、Yssc=4.7644× 1 0 5+9.4346× 1 0 5Xssc(r =0 .9992 )、Yssd=3 .40 31× 1 0 5+1 .352 2× 1 0 6 Xssd(r=0 .9992 ) ;加样回收率分别为 (93 .2± 3 .5) %、(95 .9± 1 .7) %、(95 .6± 3 .3) % (n =5)。结论 :本研究建立的柴胡皂苷a、c、d含量测定方法简便易行 ,结果准确。Objective: To establish the determination of saikosaponin a,c,d in Bupleurum Chinense DC. from 20 different areas. Method: HPLC was performed on ODS C 18 column. The mobile phase was acetonitrile H 2O with a gradient program and detected at 210nm. Results: The regression equations of Saikosaponin a, c, d were Y ssa =2.0377×10 5+1.1555×10 6X ssa ( r = 0.9999 ),Y ssc =4.7644×10 5+9.4346×10 5X ssc ( r =0.9992),Y ssd =3.4031×10 5+1.3522×10 6X ssd ( r =0.9992) respectively, and the average recovery rates were (93.2±3.5)%,(95.9±1.7)% and (95.6±3.3)% ( n =5), respectively.Conclusion: This method was simple and easy to perform and the results obtained were reliable and suitable.

关 键 词：北柴胡 柴胡皂苷 HPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]