神经营养素受体在人外周血T细胞中表达的研究  

Expression of neurotrophic receptors on T-cells in human peripheral blood

作　　者：王堰婷[1] 林琳[1] 富赛里[1] 陆佩华[1] 

机构地区：[1]上海第二医科大学神经生物学实验室,上海200025

出　　处：《细胞与分子免疫学杂志》2002年第3期214-216,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：上海市自然科学基金资助 No .992B14 0 2 2

摘　　要：目的　研究人外周血CD4 + /CD8+ T细胞 4种神经营养素受体基因的转录。方法应用尼龙毛法分离出T细胞 ,磁式细胞分离法 (MACS)分离CD4 + /CD8+ T细胞亚群 ,再以RT PCR法研究 4种神经营养素受体在两种T细胞亚群上的表达。结果未经刺激的CD4 + /CD8+ T细胞亚群不表达任何神经营养素受体。经PHA或PPD刺激后 ,CD4 + /CD8+ T细胞亚群表达trkA ,CD8+ T细胞亚群表达trkC ;而在各种状态下的T细胞上均未见表达trkB及 p75 NGFR。结论神经营养素受体在两种T细胞亚群中有不同的表达格局 ,提示不同T细胞亚群受神经营养素调节的模式可能各不相同。Aim To study the expression pattern of neurotrophic receptors on peripheral T lymphocytes. Methods Nylon column and MACS were used to isolate CD4 + /CD8 + T lymphocytes. The expression of neurotrophic receptors was detected by RT PCR. Results No neurotrophic receptor was expressed on unstimulated T lymphocytes. CD4 + and CD8 + T lymphocytes expressed trkA mRNA after activation with PHA or PPD. Moreover, CD8 + T lymphocytes expressed trkC mRNA after either stimulation. Neither PHA nor PPD activation induced the expressions of trkB and p75 NGFR . Conclusion The expression pattern of neurotrophic receptors on peripheral CD4 + and CD8 + T lymphocytes is different, suggesting that modulation of neurotrophin on CD4 + T subgroup may be distinct from that of CD8 + T subgroup by modulation.

关 键 词：CD4^+/CD8^+T淋巴细胞 MACA RT-PCR 神经营养受体 

分 类 号：R392.1[医药卫生—免疫学]