冠心病患者纤维蛋白原Bβ启动区基因多态性  

Study on the Promoter Region of Bβ-Fibrinogen Gene Polymorphism in Patients with Coronary Heart Disease

作　　者：龚五星[1] 蔡月明[1] 彭健[2] 彭澍[2] 曾志为[2] 

机构地区：[1]暨南大学医学院附属第三医院珠海市人民医院分子生物学中心,广东省珠海市519000 [2]暨南大学医学院附属第三医院珠海市人民医院心内科,广东省珠海市519000

出　　处：《中国动脉硬化杂志》2002年第2期140-143,共4页Chinese Journal of Arteriosclerosis

基　　金：珠海市科委重点科技攻关项目 (199917)

摘　　要：探讨纤维蛋白原Bβ基因启动区的两个多态性位点Bβ 14 8C T和 4 5 5G A与冠心病的相关关系。用聚合酶链反应—限制片长多态性方法对冠心病组 (14 8例 )和健康对照组 (173例 )进行了研究 ,发现Bβ 14 8T和 4 5 5A等位基因频率在冠心病组中高于对照组 (P <0 .0 5 ) , 14 8CT及 4 5 5GA基因型频率在冠心病组高于对照组 (P <0 .0 5 ) ,不同基因型血浆纤维蛋白原水平有差异 , 14 8CC和 4 5 5GG < 14 8CT和 4 5 5GA < 14 8TT和 4 5 5AA。冠心病患者组血浆纤维蛋白原水平高于对照组 (P <0 .0 1) ,两组人群同一基因型比较 ,冠心病患者组纤维蛋白原水平均高于健康对照组。表明纤维蛋白原Bβ基因启动区 14 8C T和 4 5 5G A多态性与冠心病的发生相关 ,并可影响血浆纤维蛋白原水平 ;在中国南方人群中纤维蛋白原Bβ 14 8C T和Bβ 4 5 5GAim To investigate the relationship between the promoter region of Bβ fibrinogen (Fg) gene polymorphism 148C/T, 455G/A and coronary heart disease (CHD). Methods By Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) restriction enzyme digestion methods,the genotypes and allele frequencies of Bβ fibrinogen gene polymorphism 148C/T, 455G/A were determined in 148 CHD patients and 173 controls. Result Fg Bβ 148T, 455A allele frequencies are higher in CHD group than that in control group (P<0.05). Both heterozygous genotype frequencies of 148CT and 455GA are higher in CHD group than that in control group. The plasma Fg levels have differences among genotypes: 148CC, 455GG< 148CT, 455GA< 148TT, 455AA respectively. The plasma Fg levels are higher in CHD group than that in control group, when compared the same genotype, CHD group Fg levels also higher than that of in control group. Conclusion The promoter region of Bβ Fg gene polymorphism 148C/T, 455G/A are associated with the development of CHD, and at same time impact on the plasma Fg concentration. The polymorphism 148C/T and 455G/A are complete linkage disequilibrium in Southern Chinese.

关 键 词：纤维蛋白原 基因表达 多态性 限制片长 冠状动脉疾病 

分 类 号：R541.4[医药卫生—心血管疾病]