瑞香素体外抗疟作用与其铁螯合能力的关系(英文)  

In vitro Antimalarial Effect of Daphnetin Relating to Its Iron-chelating Activity

作　　者：牟凌云[1] 王琴美[1] 倪奕昌[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》2002年第2期83-85,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金 (No .39970 669)~~

摘　　要：目的　研究瑞香素体外抗疟作用与其铁螯合能力的关系。　方法　在恶性疟原虫FCC1株常规方法体外培养中检测瑞香素和去铁胺杀裂殖体活性。利用荧光探针calcein测定瑞香素和去铁胺的铁螯合能力。　结果　与强铁螯合剂去铁胺相比 ,瑞香素具有中度的铁螯合能力。体外实验中瑞香素在 0～ 12 μmol/L剂量范围内有剂量依赖性的杀裂殖体活性。瑞香素与Fe2 + 按 2∶1混合后 ,抗疟作用明显下降。　结论　瑞香素的抗疟作用与它的铁螯合能力有关。Objective To investigate the in vitro antimalarial effect of daphnetin relating to its iron-chelating activity. Methods Schizontocidal activity of daphnetin and desferrioxamine B was tested through an in vitro assay based on the routine in vitro cultivation of Plasmodium faciparum FCC1 strain. The iron-chelating ability of each was measured by the fluorescent probe calcein. Results Daphnetin exhibited a modest iron-chelating ability compared with the powerful iron-chelator desferrioxamine B. In vitro test at the range of 0-12 μmol/L daphnetin showed a dose-dependent schizontocidal activity which could be inhibited if mixed with Fe 2+ in a ratio of 2∶1. Conclusion The dose-dependent antimalarial activity of daphnetin is related to its iron-chelating activity.

关 键 词：铁螯合能力 恶性疟原虫 瑞香素 去铁胺 CALCEIN 体外抗疟作用 

分 类 号：R382.31[医药卫生—医学寄生虫学]