山西省快生型大豆根瘤菌资源调查和鉴定  

RESOURCES INVESTlGATION AND IDENTIFICATION OF S.FREDII OF SHANXI PROVINCE

作　　者：冯瑞华[1] 徐玲玫[1] 樊蕙[1] 葛诚[1] 

机构地区：[1]中国农业科学院土壤肥料研究所

出　　处：《土壤学报》1991年第1期73-82,共10页Acta Pedologica Sinica

基　　金：国家自然科学基金

摘　　要：从山西省主要大豆产区的不同土壤和大豆品种中分离得到的38个快生型大豆根瘸菌株的鉴定表明,这些分离物的IAR除了氨苄青霉素外,均较慢生型为低。38个菌株被分为4个血清型,其中2个为新发现的,命名为2077和2120型。细胞成分N%含量为2.01-3.78,C%含量为50.52—55.53%,N/C值<10。所检测的7个菌株都有1—2个大质粒,且每个均有112 Md的大质粒。分离株的共生效应和结瘤竞争由于大豆品种不同而有显著差异。本研究表明,我国大豆起源地之一的山西省,快生型大豆根瘸菌的分布广泛,分离频率较高,菌株类型也多。This paper reports the results of biochemical and serological characteristic identification of thirty-eight fast-growing soybean rhizobia which were isolated from different soils and soybean cultivars of main soybean-prodtlcing are as in Shanxi Proyince.The cell cornporient analysis,plasmid identification,and symbiotic effectiveness and competitive nodulation tests of some strains were carried out.The results showed that all of the isolated 38 strains were typical fast-growing rhizobia.The intrinsic antibiotic resistance of the isolated strsins were lower than that of Bradyrhizobium japonicum strains except to ampicillin.By cross-agglutination reaction and fluorescent antibody technique,38 isolated strains were divided into 4 serotypes,of which two were new serotypes and named Serovar 2077 and Serovar 2120.Nitrogen and carbon contents of cell component were 2.01—3.78%and 50.52—55.53%respectively.The ratio of nitrogen to carbon was smaller than tell.Seven strains detected all contained one to two large plasmids and had one plasmid with a molecular weight of 112 Md or so.Because soybean cultivars were different,the symbiotic effectivelless and competitive nodulation of the isolated strains had distinet difference.The isolat ed strains were superior on soybean cultivar“Edou-2”to“Hefeng-29”in the symbiotic effecti veness.The dry Weight and total nitrogen of plant of at least three strains were similar to those of USDA110. When the isolates and slow-growing soybean rhisobia were mixed,the nodulation ratios of different soybean cultivars varied greatly.The nodulation ratio of fast-growing soybean rhizobia is far higher on boybean cultlvat“Houzimao”than on cultivar“Hefend'.Research results show that there is a wide distribution of fast-gwoing sovbean rhisobia in Shanxi province which is one of Chinese soybean origins.The isolation frequency is rather high,and there are more strain types in these isolates.

关 键 词：快生型 大豆 根瘤菌 鉴定 山西 

分 类 号：S153.381[农业科学—土壤学]