一种快速的活菌计数新方法研究  

STUDIES ON A NEW METHOD FOR COUNTING LIVING BACTERIAL CELL NUMBER

作　　者：魏鸿刚[1] 李元广[1] 刘健[1] 沈国敏[1] 武济民[1] 

机构地区：[1]华东理工大学海洋生化工程研究所生物反应器工程国家重点实验室,上海200237

出　　处：《微生物学通报》2002年第2期89-93,共5页Microbiology China

基　　金：上海市教育委员会重点学科研究资助项目

摘　　要：四唑盐（MTT）比色法是一种检测动物细胞活细胞数的方法。通过改变比色反应温度、反应时间和比色波长,建立了一种快速、准确的活菌计数新方法并将其应用于细菌PBW1培养过程中活菌浓度的测定。结果表明,新方法与平板稀释法的测定结果一致,且具有快速、方便等优点。MTT Colorimetric method is usually applied for measuring the living animal cell number. By changing the reaction temperature and the reaction time as well as the colorimetric wavelength, the improved MTT colorimetric method was established to count the living bacterial cell number. This new method was used to measure the living cell concentration in the process for culturing bacteria PBW1. The results measured by the improved MIT colorimetric method and dilute plate method are similar. Compared with other methods including the dilute plate method, the improved MTT colorimetric method has many advantages such as accuracy, quickness.

关 键 词：四唑盐比色法 活菌计数 快速计数 

分 类 号：Q93-3[生物学—微生物学]