铜绿假单胞菌噬菌体的分离鉴定及耐噬菌体突变频率测定  

ISOLATION AND IDENTIFICATION OF PSEUDOMONAS AERUGINOSABACTERIOPHAGE AND DETERMINATION OF PHAGE-RSISTANCEMUTATION FREQUENCE

作　　者：张克斌[1] 陈志瑾[1] 金晓琳[1] 饶贤才[1] 胡福泉[1] 

机构地区：[1]第三军医大学基础部微生物学教研室,重庆400038

出　　处：《微生物学通报》2002年第1期40-45,共6页Microbiology China

基　　金：国家自然科学基金资助项目(No.30070037)~~

摘　　要：　用铜绿假单胞菌为宿主菌自污水中分离到3株不同的铜绿假单胞菌噬菌体,命名为PaP1、PaP2及PaP3者均为DNA双链噬菌体,基因组大小分别约为47kb、34kb及24kb。3株噬菌体原液滴度（pfu）分别为109/mL、1011/mL和1011/mL。PaP1为裂菌性噬菌体,PaP2及PaP3为溶原性噬菌体。电镜观察,3株噬菌体头部均为多面体立体对称颗粒,直径分别约为70nm、55nm和65nm。PaP1属肌尾噬菌体科,PaP2和PaP3属短尾噬菌体科。研究中还发现了铜绿假单胞菌的耐噬菌体现象及耐受菌与敏感菌之间的“菌群交替”现象,经测定铜绿假单胞菌耐噬菌体的突变机率在1.4×10-7～7.9×10-7之间。Three bactreiophages of Pseudomonas aeruginosa were isolated from sewage and named as PaP1, PaP2 and PaP3. All belong to double-strand DNA phages, their genome is about 47kb, 34kb and 24kb respectively. The titre (pfu/mL) of three phages is respectively 109, 1011 and 1011, PaP1 is lytic phage, both PaP2 and PaP3 are lysogenic. Under electron microscope, All show icosahedral heads with diameter of 70nm, 55nm and 65nm respectively. PaPl belongs taxonomically to Myoviridae, and both of PaP2 and PaP3 belong to Pedoviridae. The phage-re-sistance and substitution phenomenon of the resistant flora for the sensitive were observed, and the mutation frequence of Pseudomonas aeruginosa resistant to the phage is about 1.4 × 10-7 ~ 7.9 ×10-7 determined by end-point -titer method.

关 键 词：铜绿假单胞菌 噬菌体 分离鉴定 噬菌体耐受性 

分 类 号：Q93-331[生物学—微生物学]