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机构地区:[1]中山医科大学中山眼科中心,广州510060 [2]美国辛辛那提大学儿童医院人类遗传学研究室
出 处:《神经解剖学杂志》2002年第1期8-12,W003-W004,共7页Chinese Journal of Neuroanatomy
基 金:广东省自然科学基金 (No.990 0 96)资助项目
摘 要:为了研究正常发育和单眼视觉剥夺大鼠视皮层 ERK1/ ERK2基因蛋白质表达的变化 ,本研究建立了 SD大鼠单眼视觉剥夺模型 ,以多克隆抗 ERK1/ ERK2抗体和单克隆抗双磷酸化 ERK抗体检测出生后第 1、14、2 1、2 8、45 d和成年 (90 d)正常发育和单眼视觉剥夺 (14~ 45 d)视皮层 ERK1/ ERK2蛋白表达和活性改变。结果表明 ,出生后大鼠视皮层 ERK1/ ERK2蛋白表达逐渐增加 ,至 45 d达到高峰 ,此后下降至成年达稳定水平。磷酸化 ERK蛋白表达仅见于成年大鼠视皮层。单眼视觉剥夺导致ERK1/ ERK2蛋白表达减少。提示 ERK的蛋白表达依赖于正常的视觉输入信号的刺激 ,异常的视觉经验造成表达的明显下调 ,阻断正常的发育进程。说明 ERK1和To investigate ERK1/ERK2 protein expression in visual cortex in normal developmental and deprived rats. Sprague-dawley rats underwent momocular deprivation from postnatal day 1 to 45. Sections of paraffin embeded visual cortex were obtained from the monocularly deprived group and the normal groups from postnatal day 1 to day 90(adult). Immunohistochemical analysis with anti RK1/ERK2 and anti active ERK antibody were performed on above sections to examine the expression and phosphorylaed ERKs. ERK1/ERK2 protein expressions in the rat postnatal visual cortex were increased with peak at postnatal day 45 and decreased to adult level at postnatal day 90. Phosphorylated ERK protein was found in visual cortex of adult rat. ERK1/ERK2 protein was low expressed in deprived visual cortex. ERKs protein expression was regulated by normal visual input during development. Abnormal experience leads to a downregulation of ERK1/ERK2 protein expression and interrupts normal process of development of the visual cortex. These observations suggest that ERK1/ERK2 may involve in the regulation of the developmental plasticity of visual cortex in rat during the critical period.
关 键 词:细胞外信号调节激酶系统 正常发育 大鼠 视皮层蛋白 表达 ERK1/ERK2 单眼剥夺 免疫组织化学
分 类 号:R339[医药卫生—人体生理学]
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