人BDNF成熟肽基因克隆及序列分析(英文)  

MOLECULAR CLONING AND SEQUENCING OF HUMAN BDNF GENE ENCODING MATURE PEPTIDE

作　　者：马东亮[1] 任惠民[1] 胡海涛[1] 刘勇[1] 陈新林[1] 刘朝辉[1] 李卫敏[1] 

机构地区：[1]西安交通大学医学院解剖学教研室,西安710061

出　　处：《神经解剖学杂志》2002年第1期75-78,共4页Chinese Journal of Neuroanatomy

摘　　要：为研究人脑源性神经营养因子在大肠杆菌中的表达及其在治疗 Alzheimer病中的作用 ,本文作者等克隆了其成熟肽基因并进行了序列分析。提取健康成人末梢血白细胞基因组 DNA作为模板 ,应用 PCR技术扩增人脑源性神经营养因子成熟肽基因片段 ,并将其插入 p GEM-T质粒。限制性内切酶鉴定重组质粒并进行序列测定和分析。与 Gen Bank提供的已知序列(M61181)比较 ,所克隆的人脑源性神经营养因子成熟肽基因片段长 3 5 7bp,序列完全相同。人脑源性神经营养因子成熟肽基因的克隆 ,为其在原核细胞中的表达。In order to study the expression of BDNF in E. Coli and the roles of BDNF in treatment of Alzheimer's disease, the human BDNF gene encoding mature peptide was cloned and sequenced. The genomic DNA was isolated from the human leucocytes. The PCR amplification of the BDNF gene was performed. The fragment of resulting gene was then cloned into pGEM T vector. The recombinant pGEM T plasmid was analyzed and identified by the restriction enzymes, then was sequenced. The results indicated that both the cloned DNA sequence and the GenBank (M61181) sequence were completely identical, 357bp length. It was suggested that the human BDNF gene encoding mature peptide be cloned, which provides the basis for expression in prokaryotic cells, preparing antibody and the treatment for the nervous system disease.

关 键 词：脑源性神经营养因子 聚合酶链反应 T-载体 基因克隆 人 

分 类 号：R346[医药卫生—基础医学]