南方鲇生长激素完整cDNA的克隆及其DNA序列分析  

CLONING AND SEQUENCING OF FULL LENGTH cDNA IN SILURUS MERIDIONALIS GROWTH HORMONE

作　　者：宋平[1] 向筑[1] 潘云峰[1] 胡隐昌[2] 胡珈瑞[3] 

机构地区：[1]武汉大学生命科学学院,武汉430072 [2]中国水产科学研究院珠江水产研究所,广州510380 [3]武汉大学中南医院,武汉430071

出　　处：《水生生物学报》2002年第3期262-280,共19页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(项目号:39770591);农业部重点实验室研究基金;第三世界科学院(意大利)研究基金(基金号:95-348RG/BIO/AS)资助

摘　　要：采用RT-PCR法和3'、5'RACE(Rapid amplification of cDNA ends)法从南方鲇脑垂体RNA克隆出生长激素(Growth hormone,GH)cDNA。此cDNA全长1087nt(含 Poly(A)》,其5'端非编码区长52nt、阅读框(Open reading frame,ORF)长603nt、3'端非编码区长415nt。其推导的GH由200个氨基酸组成,其中前24个氨基酸为信号肽部分。氨基酸序列比较表明,南方鲇GH与Pangasianodongigas、Ictaluruspunctatus和Heteropneustes fossilis三种鲇类的同源性分别为97.5%和95.0%,与鲢、鳙、草、鲤和鲫鱼的GH同源性达76.0%以上,而与人的GH(Ⅰ、Ⅱ)同源性最低为31.0%和29.5%.The full length cDNA that encodes Siulurus meridionalis growth hormone (SmGH) was cloned from pituritary RNA with RT-PCR、3' and 5' RACE (rapid amplification of cDNA ends). The cDNA, about 1087nt long, had a coding sequence, 5'-and 3'-untranslated regions and a poly (A) chain. The cDNA could encode a prehormone of 200 amino acid (aa) including a putative signal peptide of 24aa. The primary structure of SmGH was compared with those of other 15 species growth hormones.

关 键 词：RT-PCR 南方鲇 生长激素 CDNA 克隆 DNA序列分析 

分 类 号：Q959.4[生物学—动物学] Q575.11