中国6个人群中Y染色体15个双等位基因标记变异频率分布及单体群分析  

Y Chromosome Variations and Hyplogroups from 15 Biallelic Markers in Six Chinese Populations

作　　者：于敏[1] 张咏莉[1] 陈峰[1] 薛雅丽[1] 于旸[1] 马琳琳[1] 黄小义[1] 刘岸[1] 史榕茜[1] 吕芙蕖[1] 黄承滨[1] 张贵寅[1] 李璞[1] 傅松滨[1] 

机构地区：[1]哈尔滨医科大学生物教研室与医学遗传学研究室,哈尔滨150086

出　　处：《Acta Genetica Sinica》2002年第4期283-289,共7页

基　　金：国家自然科学基金 (项目号 :3 93 92 90 0 )~~

摘　　要：以 6个群体 (5个民族 ) 342名男性为研究对象 ,采用等位基因特异性PCR技术对Y染色体上 15个双等位基因标记进行基因分型 ,得到 15个标记的变异频率分布并界定了 6个群体的单体群。结果显示 ,两个汉族人群中M9G的频率相当高 (96 2 0 %和 96 4 3% ) ,为一特征性标志 ;四川汉族以高M95T(82 14 % )为显著特点 ;回族以M4 5A(18 5 7% )的高频率有别于其他 5个群体 (0 % )。进一步对单体群分析表明 ,4个少数民族群体享有共同的基本单体群 ,并根据群体间的共有单体群比较推测出 4个少数民族间的相对遗传距离 。By using 15 biallelic markers, 342 male individuals from six populations in China were genotyped with ASPCR (allele specific PCR). The 15 biallelic markers included M1 (YAP), M15 (9bp insertion), M89 (C→T),M9 (C→G), M119 (A→C), M50 (T→C), M110 (T→C), M103 (C→T), M95 (C→T), M88 (A→G), M111 (2 bp deletion), M45 (G→A), M122 (T→C), M7 (C→G) and M134 (1bp deletion). The distribution of variation frequencies of 15 biallelic markers in six populations showed that with the extremely high frequencies of M9G (96 20% & 96 43%) and Han nationality displayed higher diversity than the four minority populations. Its noteworthy that M95T (82 14%) in Sichuan Han and M45A (18 57%) in Hui gave prominance to the two populations. The six populations displayed 34 (Fujian Han), 21 (Sichuan Han), 14 (Mongol), 26 (Hui), 10 (Xibo) and 8 (Hezhe) haplogroups respectively with 2, 1, 2, 1, 2 and 2 prominent haplogroups among them. Furthermore, the haplogroup analysis revealed that one predominant haplogroup was shared in the four minority populations and even two predominant haplogroups were shared in Mongol, Hezhe and Xibo. Unlike Han populations, the minority populations showed strikingly different haplogroups which were close to the ancestral pattern. However, the two Han populations exhibited divergence between them with the distinct frequencies of M89T and M95T. With the comparison of the number of people sharing the common haplogroups between any two of the four minority populations, relative genetic ditance among them was deduced.

关 键 词：中国 基因标记 变异频率分布 Y染色体 双等位基因 单体群 

分 类 号：Q987[生物学—遗传学]