刺激室旁核及加压素对大鼠胃缺血-再灌注损伤的保护作用  

Protective effects of paraventricular nucleus stimulation and vasopressin on gastric ischemia-reperfusion injury in rats

作　　者：张建福[1] 张咏梅[2] 阎长栋[1] 周秀萍[3] 祁友键[2] 

机构地区：[1]徐州医学院生理学教研室神经生物学教研室,徐州221002 [2]徐州医学院生理学教研室 [3]徐州医学院神经生物学教研室,徐州221002

出　　处：《生理学报》2002年第2期133-138,共6页Acta Physiologica Sinica

基　　金：ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No 3 9970 2 81)andJiangsuProvinceEducationMinistry(No 99KJB3 10 0 0 5 )

摘　　要：采用夹闭大鼠腹腔动脉 30min ,松开动脉夹血流复灌 1h的胃缺血 再灌注损伤 (gastricischemia reperfu sioninjury ,GI RI)模型 ,观察了电或化学刺激室旁核 (paraventricularnucleus,PVN)及外源性加压素 (arginine vasopres sion ,AVP)对GI RI的影响 ,并对PVN的调控通路进行了初步分析。结果表明 :电或化学刺激PVN后 ,GI RI显著减轻 ;损毁双侧孤束核 (nucleustractussolitarius,NTS)或一侧NTS内注射AVP V1受体阻断剂 ,均能取消电刺激PVN对GI RI的效应 ;去除脑垂体后不影响PVN的作用 ;切断膈下迷走神经或切除腹腔交感神经节 ,则能加强电刺激PVN对GI RI的影响 ;PVN内注射不同剂量的AVP同样能减轻大鼠GI RI损伤。结果提示 :PVN及AVP对大鼠GI RI具有保护作用 ;PVN的这种作用可能是因电或化学刺激后 ,激活了其中的加压素能神经元 ,经其下行投射纤维释放AVP作用于NTS神经元的AVP V1受体 ,并通过迷走和交感神经介导 ,从而影响GI RI;而似与PVNThe effects of paraventricular nucleus (PVN) stimulation and vasopressin on gastric ischemia reperfusion injury (GI RI) were investigated in male SD rats of which the celiac artery was clamped for 30 min and reperfused for 1 h by removal of the clamp. The results were as follows. Both electrical and chemical stimulation of the PVN obviously attenuated the GI RI. Bilateral electrolytic lesion of the nucleus tractus solitarius (NTS) or microinjection of AVP V 1 receptor antagonist into the NTS could eliminate the protective effect of electrical stimulation of the PVN on GI RI. Hypophysectomy did not influence the effect of electrical stimulation of the PVN. Both vagotomy or sympathectomy could increase the effect of stimulating PVN on GI RI. Microinjection of arginine vasopressin (AVP) into the PVN also attenuated the effect on GI RI. These results suggest that the PVN and AVP participate in the regulation of GI RI and play an important role in protection against GI RI. This protective effect of PVN on GI RI might be mediated by activation of AVP ergic neurons in the PVN, which release AVP from the descending projection fibers and activate the AVP V 1 receptors on the NTS neurons. The vagus and sympathetic nerves are involved in the efferent pathway exerting their effects on GI RI. Hypophysis does not seem to be involved in the protective effect of PVN stimulation.

关 键 词：刺激 室旁核 加压素 大鼠 胃缺血-再灌注损伤 保护作用 孤束核 脑垂体 

分 类 号：R338[医药卫生—人体生理学]