超广谱β-内酰胺酶细菌检测方法及与耐药质粒关系研究  

DEVELOPMENT OF A NOVEL TEST IDENTIFYING EXTENDED-SPECTRUMBETA-LACTAMASE PRODUCING STRAINS RELATIVE TO RESISTANT PLASMIDS

作　　者：苑广盈[1] 赵学印[1] 刘安蓉[1] 吴耀光[1] 申丽[1] 王永瑞[1] 

机构地区：[1]泰山医学院第二附属医院,山东济南250031

出　　处：《中国微生态学杂志》2002年第2期89-90,共2页Chinese Journal of Microecology

摘　　要：目的 :建立一种准确、快速、低廉的检测超广谱β-内酰胺酶 (ESBL )细菌方法。方法 :以克拉维酸为 ESBL 的抑制剂、3种第三代头孢菌素 (TGC)和氨曲南为底物、某些药敏结果为补充 ,建立了多底物协同方法 ,并与 E- test方法进行了对照 ,符合率 80 % ,不符合菌株用质粒提取、细菌转化、接合传递试验进行验证。结果 :对 116株疑产 ESBL细菌用多底物协同方法和 E- test方法同时检测 ,前者比后者阳性率高。结论 :该方法准确、快速、价格低廉 ,特别适于常规应用。Objective:To develop a reliable and time and cost saving assay to identify extended-spectrum beta-lactamase(ESBL) producing strains.Methods:A poly-substrate synergy method(PSSM) was developed with clavulanic acid as ESBL inhibitor,aztreonam and three third-generation cephalosporins as substrates and some established drug sensitive results as references.Results of PSSM were compared with that of E-test and the two methods resulted in conformity with each other in 80% of the strains tested.Strains with different results were further subjected to plasmid extraction,plasmid transformation and conjugation procedures for final identification.Result:Among the 116 strains of ESBL producing suspects tested by both the methods a greater positive rate was achieved by PSSM than that by E-test.Conclusion:PSSM is a reliable,fast and low-cost method for ESBL bacteria identification suitable for routine practice.

关 键 词：超广谱Β-内酰胺酶 细菌 检测方法 耐药质粒 

分 类 号：R978.11[医药卫生—药品] R378[医药卫生—药学]