应用原位杂交技术检测感染中国对虾和克氏原鳌虾体内的白斑综合征病毒  

Detecting White Spot Syndrome Virus by In Situ Hybridization

作　　者：雷质文[1] 黄倢 杨冰[1] 史成银[1] 俞开康[2] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]青岛海洋大学水产学院,山东青岛266003

出　　处：《中国预防兽医学报》2002年第3期227-230,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家重点基础研究规划项目 (G19990 12 0 0 2 );"九五"攻关项目"(96_0 0 5_0 3_0 1_0 2 )资助

摘　　要：用PCR制备地高辛 (DIG)标记探针 ,采用原位杂交技术检测感染中国对虾和克氏原鳌虾体内的白斑综合征病毒(WSSV)。患病严重的濒死中国对虾的角化上皮、胃上皮、触角腺、肝胰腺上皮、疏松结缔组织、肌肉、造血组织、鳃、卵巢之结缔组织细胞和滤泡细胞、精巢之结缔组织细胞原位杂交呈阳性 ;人工注射感染克氏原螯虾的角化上皮、胃上皮、肝胰腺上皮、疏松结缔组织、肌肉、造血组织、鳃原位杂交检测呈阳性。PCR制备DIG标记探针与健康中国对虾和克氏原螯虾无交叉反应 。In the present study,a DIG labeled WSSV DNA probe produced by PCR was used to detect WSSV by in situ hybridization,(1)WSSV_positive cells were observed in cuticular epidermis,stomach epithelium,antennal gland ,gill,hematopoietic tissue,loose connective tissue,hepatopancreas epithelium of Penaeus chinensis.Follicle cells and connective cells were WSSV positive by in situ hybridization in the ovaries of Penaeus chinensis.Connective cells were WSSV positive by in situ hybridization in the testis of Penaeus chinensis.(2)WSSV_positive cells were observed in cuticular epidermis,stomach epithelium,gill,hematopoietic tissue,loose connective tissue,hepatopancreas epithelium of artificially infected Procamburas clarkii.

关 键 词：应用 原位杂交技术 中国 对虾 克氏原鳌虾 白斑综合征病毒 病毒感染检测 

分 类 号：S945.1[农业科学—水产养殖]