APP17肽对DM大鼠海马神经元胰岛素和凋亡信号传导通路相关蛋白的影响  

Effect of APP 17-mer Peptide on some proteins related to insulin and apoptosis signal transduction pathways in the hippocampus of diabetic rat brain

作　　者：左晓虹[1] 姬志娟[1] 艾厚喜[1] 赵咏梅[1] 赵志炜[1] 盛树力[1] 

机构地区：[1]首都医科大学宣武医院北京老年病医疗研究中心,邮政编码100053

出　　处：《中国糖尿病杂志》2002年第2期85-88,共4页Chinese Journal of Diabetes

基　　金：国家科技部 973项目资助 ;项目编号为 G2 0 0 0 0 570 0 5

摘　　要：目的　观察糖尿病大鼠海马脑区胰岛素和凋亡信号传导通路中某些相关蛋白的表达及 APP17肽对其表达的影响。方法　链脲佐菌素诱发大鼠糖尿病模型 ,并用 APP17肽治疗。用免疫沉淀和 Western- blot法分析海马中信号传导及部分凋亡相关蛋白 ;电镜观察大鼠脑组织超微结构。结果　 DM大鼠 IGF- IRα、Akt/ PKB、CREB表达明显降低 ,APP17肽治疗后明显上调 (P <0 .0 5 ) ,GSK- 3β、PP- 1及凋亡相关蛋白 Bax、AIF表达增加 ,治疗后则明显降低 (P <0 .0 5 ) ;超微结构表明 APP17肽治疗后兴奋性突触小泡明显减少。结论　糖尿病大鼠海马脑区胰岛素和凋亡信号传导通路中某些重要蛋白表达异常 ,APP17肽部分纠正这些蛋白的异常表达 ,这可能是其保护Objective To investigate the effect of APP 17 mer Peptide on some proteins related to insulin and apoptotic signal transduction pathways in the hippocampus of diabetic rat brain.Methods The diabetic rat model was produced by intraperitoneal injection of streptozotocin and some of the diabetic rats were treated by APP 17 mer peptide. Immunoprecipitation and Western blot were used to analyse the proteins related to the insulin and apoptosis signal transduction pathway. Electron microscope was used to examine the ultrastructural change of the hippocampal neurons.Results In the hippocampus of the diabetic brain, IGF IRα, Akt/PKB and CREB involved in the insulin transduction pathway were decreased, while GSK 3β, PP 1, Bax and AIF related to apoptosis signal transduction pathway were increased. In addition, the hippocampal neurons of the DM rat showed degenerative changes. APP 17 mer peptide partially corrected the changes of the above protein and improved the degenerative changes of the hippocampal neurons. Conclusion Some of the important proteins are abnormally expressed in the insulin and apoptosis signal transduction pathways in the hippocampus of diabetic rat brain. APP 17 mer peptide can partially correct the abnormal expression, which may be related to its protective effect on hippocampal neurons of the DM rat.

关 键 词：DM APP17肽 神经元信号传导途径 凋亡相关蛋白 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]