气相色谱法测定鱼油多烯乙酯的含量  

Determination of fish oil ethyl polyenoic acid by gas chromatography

作　　者：李群[1] 周淑平[1] 丁锐[1] 

机构地区：[1]北京市药品检验所,北京100035

出　　处：《中国生化药物杂志》2002年第2期72-74,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的用气相色谱法测定鱼油多烯乙酯中二十碳五烯酸乙酯 (EPA E)和二十二碳六烯酸乙酯 (DHA E)的含量。方法采用气相色谱法 ,聚二乙二醇酯为固定相 ,涂布浓度为 10 % ,载体为ChromosorbWAWDMCS 80～ 10 0目的气相柱。结果EPA E平均回收率为 10 0 .31% ,RSD为 2 .5 4 % ;DHA E平均回收率为 10 3.37% ,RSD为 2 .76 %。结论此法操作简便、准确、重现性、稳定性好 。PurposeThe aim is to determine the eicosapentaenoic acid ethyl ester and docosahexaenoic acid ethyl ester in fish oil ethyl polyenoic acid by gas chromatography (GC).MethodsGas chromatography, stationary phase: Chrosorb W AW DMCS 80～100 mesh coated with 10% DEGS.ResultsThe EPA E recovery was 100.31% and the RSD was 2.54%. The DHA E recovery was 103.37% and the RSD was 2.76%.ConclusionThis method was convenient,accurate reproducible and stable,so it was suitable for assay of eicosapentaenoic acid ethyl ester and docosahexaenoic acid ethyl ester in fish oil ethyl polyenoic acid.

关 键 词：鱼油多烯乙酯 气相色谱法 二十碳五烯酸乙酯 二十二碳六烯酸乙酯 含量 

分 类 号：R927.2[医药卫生—药学]