玉米功能短肽的制备及其类超氧化物歧化酶活性研究  

Study on the preparation of corn functional oligopeptides and its SOD-like activity

作　　者：徐力[1] 刘立[2] 李相鲁[1] 乔岩梅[1] 吴晓霞[1] 张学忠[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室 [2]白求恩医科大学制药厂,吉林长春130021

出　　处：《中国生化药物杂志》2002年第2期78-80,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的制备具有类超氧化物歧化酶 (SOD)活性的玉米功能短肽 ,并与SOD进行活性比较。方法以玉米蛋白粉为原料 ,采用酶水解的方法 ,经SephadexG 2 5分离 ,以TNBS法测定各组分的平均链长 ,用邻苯三酚自氧化法测定各组分的抗氧化活性 ,同时与SOD进行活性比较。结果含有 2～ 6个氨基酸残基的玉米功能短肽 ,对邻苯三酚自氧化具有较好的抑制作用 ,玉米功能短肽 (浓度 10mg ml)的抑制率为 2 1.78%。PurposeThe aim is to prepare corn functional oligopeptides, which has SOD like activity, and to compare the product with SOD. MethodsCorn functional oligopeptides were prepared by using a type of alkaline protease. The hydrolysis degree (DH) , antioxidative effects and molecular weight distribution were examined.ResultsCorn functional oligopeptides had high ability in inhibiting selfoxidation of pyrogallol, and the inhibition rate is up to 21.28% at the concentration of 10mg/ml .ConclusionCorn functional oligopeptides have antioxidative and SOD like activity.

关 键 词：玉米功能短肽 类超氧化物歧化酶活性 抑制率 制备 

分 类 号：Q516[生物学—生物化学] R392－33[医药卫生—免疫学]