雾化吸入α_1-抗胰蛋白酶对绿脓杆菌肺部感染大鼠的作用研究  

Effect of aerosolized α_1-antitrypsin on Pseudomonas aeruginosa pneumonia in rats

作　　者：张伟[1] 蔡映云[1] 顾宇彤[1] 张志凤[1] 于金娣[1] 陈琪[1] 祝慈芳 

机构地区：[1]复旦大学附属中山医院,上海200032 [2]上海市生物制品研究所,上海200030

出　　处：《中国危重病急救医学》2002年第5期282-284,共3页Chinese Critical Care Medicine

基　　金：卫生部科技基金资助项目 (No.98115 0 )

摘　　要：目的 :观察雾化吸入 α1 抗胰蛋白酶 (α1 AT)对绿脓杆菌肺部感染大鼠体内炎症反应的抑制作用及其机制。方法 :雾化吸入 α1 AT来干预绿脓杆菌肺部感染大鼠 ,比较干预组与对照组动物的死亡率、肺泡灌洗液 (BAL F)中细胞总数、蛋白酶 (NE)活性、白介素 8(IL 8)含量及肺内细菌总数。结果 :接种绿脓杆菌3.0× 10 6 CFU的大鼠 ,干预组动物死亡率 (8% )明显低于对照组 (5 8% ,P<0 .0 5 )。接种绿脓杆菌 2 .4× 10 6 CFU的大鼠 ,干预组 BAL F中 NE活性、IL 8含量及肺内细菌总数均较对照组显著降低 (P均 <0 .0 5 )。结论 :雾化吸入 α1 AT可抑制绿脓杆菌肺部感染大鼠肺内炎症反应程度 ,降低急性期动物死亡率及增强机体对细菌的清除力。Objective:To know if aerosolized α 1antitrypsin(α 1AT) can suppress inflammatory response and its mechanism in rats with Pseudomonas aeruginosa(PA) pneumonia.Methods:Fortyeight SD rats were involved and were divided into two groups randomly.One group was inoculated 3 0×10 6 CFU PA,the other group was 2 4×10 6 CFU.Animals were aerosolized 10 ml liquid every day,with was NS or α 1AT.Then the mortality rate,the total cell counts,the activity of NE,the capacity of IL8 in bronchoalveolar lavage fluid(BALF) and the bacterial clone forming units of lungs were compared.Results:In animals with 3 0×10 6 CFU PA inoculation,the mortality rate of the aerosolized group was 8%,which was lower than the control group(58%, P <0 05).In animals with 2 4×10 6 CFU PA inoculation,the activity of NE,the capacity of IL8 in BALF and the bacterial clone forming units of lungs in aerosolized α 1AT group were lower than those in the controls(all P <0 05).Conclusions:Aerosolized α 2AT can inhibit the inflammatory response in rats with PA pneumonia,reduce the mortality rate in acute phase,and improve the host clearance capacity to bacteria.

关 键 词：雾化吸入 Α1-抗胰蛋白酶 绿脓杆菌 肺部感染 大鼠 作用 

分 类 号：R563[医药卫生—呼吸系统]