黄山药总皂苷肠内菌代谢及代谢产物吸收的研究  

Studies on the Metabolism of DX by Intestinal Bacteria and the Absorbed Components in Serum

作　　者：马海英[1] 周秋丽[2] 王本祥[3] 

机构地区：[1]沈阳铁路局中心医院,沈阳市110005 [2]白求恩医科大学生物工程研究所,长春市130021 [3]长春中医学院新药研究中心,长春市130021

出　　处：《中国药房》2002年第4期204-205,共2页China Pharmacy

摘　　要：目的 :本文离体和整体观察人和大鼠肠内菌对黄山药总皂苷(DX)的代谢作用及整体给予DX后吸收入血的有效成分。方法 :用薄层色谱(TLC)及电喷雾质谱(ESI -MS)法检测粪中DX及其代谢产物。整体给予大鼠灌服DX900mg/kg ,于给药后不同时间采集尿及血清样品 ,用ESI -MS检测吸收入血成分。结果 :DX容易被人和大鼠消化道菌群代谢 ,随着代谢时间的延长 ,出现了各种甾体皂苷的降解产物及终产物薯蓣皂苷元 (Dio)。整体实验表明 ,在大鼠血及尿中均发现分子量为415 3的代谢产物 ,经ESI -MS二级质谱分析 ,上述分子量的化合物为Dio。结论 :DX可被人和大鼠肠内菌代谢 ,DX经口服后Dio被吸收入血。OBJECTIVE:To study the in vitro and in vivo metabolism of DX by intestinal bacteria in rats and volunteers and the active components in serum of rat after oral administration of DX 900mg/kg METHODS:To detect DX and its metabolites in stool,urine and serum with TLC and EST-MS RESULTS:In vitro,DX was decomposed easily by rat and human intestinal bacteria,and various metabolites were found With prolongation of metablism,metabolite with molecular weight of 415 3 was shown to be corresponding to diosgenin in rat serum and in urine of rats and volunteers CONCLUSION:The above-mentioned DX was decomposed easily by intestinal bacterias and diosgenin was absorbed into serum after oral administration of DX

关 键 词：黄山药总皂苷 肠内菌代谢 电喷雾质谱 薄层色谱 薯蓣皂苷元 薯蓣皂苷 中药 

分 类 号：R285[医药卫生—中药学]