广西地区汉族2型糖尿病血管并发症与血管紧张素转换酶Ⅰ基因多态性关系的研究  

Relationship between the polymorphism of angiotensin converting enzyme gene and vascular complications in type 2 diabetes in Guangxi region

作　　者：廖蕴华[1] 冼苏[2] 罗佐杰[2] 孙安远[1] 郭谊[3] 张红[4] 陈宇明[5] 

机构地区：[1]广西医科大学第一临床学院肾内科,南宁530021 [2]广西医科大学第一临床学院第一临床学院内分泌科,南宁530021 [3]广西医科大学临床检验中心,南宁530021 [4]广西医科大学生化教研室,南宁530021 [5]广西医科大学第一临床学院第一临床学院心血管科,南宁530021

出　　处：《中国实用内科杂志》2002年第5期270-272,共3页Chinese Journal of Practical Internal Medicine

基　　金：广西壮族自治区教育委员会基金资助项目 (编号 1998.1-4 8)

摘　　要：目的　探讨 2型糖尿病 (DM)及血管并发症与ACE基因插入 /缺失 (I/D)多态性的相关情况。方法　采用多聚酶链反应 (PCR)技术 ,对 12 0名广西地区汉族 2型DM组及 10 0名汉族正常对照组ACE基因I/D多态性进行检测。结果　 2型糖尿病肾病 (DN)组ACE基因D等位基因及DD基因型频率高于正常对照组 ,Ⅰ等位基因及Ⅱ基因型频率低于正常对照组 ;2型DM合并冠心病 (CHD)组ID基因型频率高于无CHD组 ,Ⅱ型基因频率低于无CHD组。 2型DM并视网膜病变 (DR)组ACE基因各等位基因及基因型频率与无DR组及正常对照组比较无统计学意义 ;2型DM并高血压组 (HP)ACE基因各等位基因及基因型频率与无HP组及正常对照组比较无统计学意义。结论　①广西地区汉族 2型DM合并DN及合并CHD与ACE基因I/D多态性有关 ;②DD基因型及D等位基因可能为 2型DM合并DN的易感基因 ,Ⅱ型及Ⅰ型等位基因可能为 2型DM合并DN的保护基因 ;ID型基因可能为 2型DM合并CHD的易感基因 ,Ⅱ型基因可能为 2型DM合并CHD的保护基因。③广西汉族 2型DM合并HP及合并DR与ACE基因I/D多态性无关联。Objectives To investigate the relationship between the insertion and deletion (I/D) polymorphism of human angiotensin converting enzyme (ACE) gene and the vascular complications in type 2 diabetes. Methods The I/D polymorphism of ACE gene was detected by polymerase chain reaction in 120 type 2 diabetic patients and 100 healthy controls. Results The frequency of DD genotype was significantly higher, while frequency of II genotype was lower in diabetic nephropathy patients (DN) than those in control group. The frequency of ID genotype was significantly higher, while frequency of II genotype was lower in type 2 diabetic patients with coronary heart disease (CHD), when compared to those without CHD. The frequencies of ACE genotypes in patients with diabetic retinopathy (DR) or hypertension (HP) were not different from those in control group and non-DR or non- HP patients. Conclusions ① There were relationships between the I/D polymorphism of ACE gene and DN and CHD in type 2 diabetes. ② The DD genotype and D allele of ACE gene might be as markers in predisposing DN, while II genotype and I allele as protective factors for DN in diabetes. The ID genotype might be as a marker in predisposing CHD, while II genotype as a protective factor for CHD in diabetes.③ There was no relationship between I/D polymorphism of ACE gene and HP or DR in diabetes in Guangxi region.

关 键 词：聚合酶链反应 血管紧张素转换酶I 广西地区 汉族 2型糖尿病 血管并发症 基因多态性 

分 类 号：R587.2[医药卫生—内分泌] R543[医药卫生—内科学]