RT-PCR快速检测口蹄疫病毒  

Rapid Detection of Foot-and-mouth Disease Virus by RT-PCR

作　　者：娄高明[1] 杜伟贤[1] 杨傲冰[1] 周秀蓉[1] 张春红[1] 谢明权[1] 

机构地区：[1]广东省兽医生物技术重点实验室,广州510640

出　　处：《畜牧兽医学报》2002年第3期308-311,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：广东省自然科学基金重大项目 (963 0 0 7) ;广东省科技攻关项目 (99B0 5 60 1G) ;广东省农科院院长基金项目 (96 基金 10 )

摘　　要：根据口蹄疫病毒VP1基因的序列 ,设计了 1对引物 ,建立了检测口蹄疫病毒的RT -PCR方法。对FMDV各毒株检测 ,结果均为阳性 ,而对猪病毒性疾病相关病毒进行检测 ,结果均为阴性 ;检测 19份已知阳性样品 ,检出率 10 0 % ;与动物接种试验比较 ,符合率 10 0 % ,证明该方法特异。与经典方法动物接种试验比较 ,其灵敏度提高10 0倍左右 ;对O3I3株的细胞毒进行检测 ,其敏感度达 10个TCID50 。初步结果表明所建立的RTOne pair of primers amplified the VP1 gene of foot and mouth disease virus(FMDV)was designed and synthesized.RT-PCR technique detected the RNA of FMDV was established after selecting the best reaction conditions.Although the positive results were obtained from FMDV F29,O3I3,MF307,T501,T509,T510,OⅡMF249 strains,the negative results were achieved from swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,pseudorabies virus.19 positive samples tested with animal experiment have also been examined by RT-PCR,the positive rate of the two methods is 100% and the same.The RT-PCR was able to detect 10 TCID 50 of FMDV O3I3 strain,at least 100 times more sensitive than animal infectivity assay.The results showed that the established FMDV RT-PCR technique provided a more sensitive,specific and reliable method for diagnosis and epizootic study of the foot and mouth disease.

关 键 词：RT-PCR 快速检测 口蹄疫病毒 

分 类 号：S852.659.6[农业科学—基础兽医学] S854.43[农业科学—兽医学]