一株假单胞菌(Pseudomonas sp.)产生的胞外脂酶  

PRODUCTION OF EXOLIPASE BY PSEUDOMONAS SP.

作　　者：朱明华[1] 李祖义[1] 杜彬[2] 周姚宇 郑伟军[2] 

机构地区：[1]中国科学院上海有机化学研究所 [2]云南省农业科学院生物工程研究室

出　　处：《微生物学报》1991年第1期48-53,共6页Acta Microbiologica Sinica

摘　　要：假单胞菌(Pseudomonas sp.)生长在一定的培养条件中能产生胞外脂酶。最适碳源为1.0%淀粉,氮源为1.0%蛋白胨。一些植物油,如橄榄油、糠油、菜油等能诱导脂酶的大量产生,诱导脂酶产生的橄榄油最适浓度为0.5%。无机离子在菌培养过程中对脂酶产率影响很大,K^+、Na^+、Mg^(2+)、Ca^(2+)等对脂酶产生有促进作用,而Mn^(2+)、Ba^(2+)、Zn^(2+)、Fe^(3+)、Co^(2+)、Cu^(2+)等则抑制脂酶产生。非离子表面活性剂(如tween、span及糖脂)能刺激胞外脂酶的产生。Uuder certain culture conditions, a strain of Pseudomonas sp. was able to proluce exolipase. The optimal medium compositions for lipase producing contains 1.0% starch (carbon source) and 1.0% peptone (nitrogen source). Some kinds of vegetable oil, such as olive oil, rice oil, rape oil could induce lipase production. High yield of exolipase was obtained when the concentration of oilve oil was 0.5%. Inorganic ions could affect the production of exolipase: K^+,Na^+, Mg^(2+),Ca^(2+), could increase the lipase activity, whereas Mn^(2+),Ba^(2+),Zn^(2+) Fe^(3+),Co^(2+), Cu^(2+) inhibited it. Non-ionic surfactants (tween, span, glycolipid) could stimulate the production of exolipase.

关 键 词：假单胞菌 胞外脂酶 

分 类 号：Q939.112[生物学—微生物学]