棒状杆菌腈水合酶的形成条件  

OPTIMUM CONDITIONS FOR NITRILE-HYDRATASE FORMATION BY CORYNEBACTERIUM ZBB-21

作　　者：李文忠[1] 张鸿翼[1] 杨惠芳[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《微生物学报》1991年第2期115-121,共7页Acta Microbiologica Sinica

基　　金：国家自然科学基金

摘　　要：本文研究了棒状杆菌(Corynebacterium)ZBB-21腈水合酶形成的最适条件。在培养基中加入Fe^(2+)、维生素B_1和L-谷氨酸等,并以n-丁腈做诱导物,可明显促进该菌腈水合酶的生物合成。ZBB-21菌在选定的培养基中,于28℃培养64小时,其腈水合酶比活力可达83.1u/mg,而酰胺酶的比活力只有1.1u/mg。腈水合酶比活力比以前报道的提高9倍。The optimum culture condition for the formation of nitrile hydratase with the highest activity by Corynebacterium ZBB-21 have been studied in this paper. Additions of ferrous ions, vitamin B_1, L-glutamic acid and the use of n-butyronitrile as an inducer greatly enhanced nitrile hydratase production. When the ZBB-21 was cultivated for 64 h at 28℃ in the chosen culture broth, the specific activity of nitrile hydratase was up to 83.1 u/ mg and that of amidase was 1.1 u/mg only. The former was about 9-times higher than that in a medium previously reported.

关 键 词：棒状杆菌 腈水合酶 

分 类 号：Q939.97[生物学—微生物学]