三角酵母二倍体菌株的选育  

THE BREEDING OF DIPLOID STRAINS OF TRIGONOPSIS VARIABILIS BY PROTOPLAST FUSION

作　　者：李维泉[1] 焦瑞身[1] 

机构地区：[1]中国科学院上海植物生理研究所

出　　处：《微生物学报》1991年第5期371-375,共5页Acta Microbiologica Sinica

摘　　要：由三角酵母(Trigonopsis variabilis)原生质体融合,获得了35株原养型融合子。通过对其中四株进行详细分析表明,融合子细胞体积和 DNA 含量为两亲株之和,苏木精染色显示单核,这些结果证明融合子为亲株的二倍体。此外,融合子的生长速度、D-氨基酸氧化酶以及细胞蛋白质含量均明显高于亲株。电镜形态观察进一步证明融合子细胞大于亲株。Fusion products were prototrophic str-ains with high stability.The cell size andDNA content were about the sum of cells ofthe two parent strains.Protein content offusion products was higher than that of pa-rents by 11—46%.The nucleus of fusionproducts was uninuceated as seen by hemato-xylin nuclear staining.Growth rate,biomassand D-amino acid oxidase activity of fusantswere higher or much higher than that of theparents.

关 键 词：三角酵母 二倍体 选育 

分 类 号：Q939.503[生物学—微生物学]