我国呼吸道合胞病毒抗原亚型的初步探讨  

PRELIMINARY STUDIES ON SUBTYPES OF RESPIRATORY SYNCYTIAL VIRUS IN CHINA

作　　者：杜金林[1] 王之樑[1] 刘成贵[1] 王芳[1] 张又[1] 张淑芳 常汝虚[2] 李桦[3] 段佩若[4] 

机构地区：[1]首都儿科研究所 [2]广州市儿童医院 [3]南京市儿童医院 [4]解放军262医院

出　　处：《微生物学报》1991年第6期488-491,共4页Acta Microbiologica Sinica

基　　金：北京市自然科学基金

摘　　要：呼吸道合胞病毒(RSV)作为婴幼儿下呼吸道感染的重要病原,早在20多年前已被证实,其分离株之间存在细微的抗原差异。随着特异的单克隆抗体(MAbs)的应用,人们在发现 RS病毒不同分离株之间主要结构蛋白组分上存在抗原变异性的同时,根据不同 RS 病毒分离株与MAbs 的反应性而将 RS 病毒分成 A、B 两个亚型。这两个抗原亚型不仅在人群中相互循环着出现。An analysis of subtypes of 9 respiratory syncytial(RS) viruses isolated from Guangzhou and Nanjing areas of china was carried out with eight Sweden RS-subtype specific monoc- lonal antibodies (MAbs) and 7 internal anti- RS MAbs.All these MAbs directed against re- spectively the large Glycoprotein (G),fusion protein (F),nucleoprotein (NP),and phosp- hoprotein (P) components of the prototype Long strain of RS virus.The patterns of the reactions of these MAbs to the nine isolated strains of RS virus were compareed with in- direct immunofluorescence assay (IFA),alka- line phosphoesterase-anti alkaline phosphoeste- rase (APAAP) enzyme-linked assay and Wes- ternblotting.(?)he antigenic variations were founded among the strains of RS virus,and two subtypes allocated to the subtype A and B of RS virus by using the eight RS-subtype specific MAbs.Seven out of the 9 isolated strains of RS virus belonged to the subtype A, and two were being to the subtype B.The antigenic diversities were also founded within the same subtype,and the main pronounced difference were observed on the G glycopro- tein by using the internal anti-RS MAbs.These findings are potentially important both for vaccine development and for the understanding of clinical and epidemiological characteristics of RS virus.

关 键 词：呼吸道 合胞病毒 抗原 亚型 

分 类 号：R373.1[医药卫生—病原生物学]