生物酶振荡器研究谷胱甘肽的抗氧化效应  

Study on the Antioxidation Effect of Glutathione Based on the Peroxidase-Oxidase Biochemical Oscillator

作　　者：甘南琴[1] 蔡汝秀[1] 林智信[1] 杨树涛[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《化学学报》2002年第4期715-719,共5页Acta Chimica Sinica

基　　金：国家自然科学基金 (No .2 0 0 75 0 18)资助项目

摘　　要：组装了一种生物酶振荡器 (Peroxidase OxidaseBiochemicalOscillator)的实验装置、建立了相应的实验方法 ,在此基础上得到单倍周期、四倍周期、混沌等丰富的振荡图谱信息 ,并采用它作为“探针” ,检测了谷胱甘肽 (GSH)的抗氧化效应 ,它可作为定量分析的基础 ,线性范围为 9.38× 10 -7～ 7.5 0× 10 -5mol·L-1,探讨了某些可能的反应机理 .Complex dynamics have become increasingly important in biochemical and physiological contexts in recent years. A good example for studying the nonlinear and non-equilibrium dynamics is the peroxidase-oxidase (PO) reaction. The PO reaction involves the oxidation of reduced nicotinamide adenine dinucleotide (NADH) by molecular oxygen and overall reaction catalyzed by horseradish peroxidase (HRP). The non-linear behavior of this reaction is usually realized in a reactor containing the HRP, methylene blue (MB), and 2,4-dichlorophenol (DCP). NADH and O 2 are supplied continuously to this reaction mixture from external sources. In this paper, the experimental system and methods of the Peroxidase-Oxidase biochemical oscillator has been set up successfully. Different kinds of oscillating waves such as simple perodic, quad-perodic, chaos and damped oscillation have been found based on this experimental system and methods. To protect the cells and organ systems of the body against reactive oxygen species, humans have evolved a highly sophisticated and complex antioxidant protection system. It involves a variety of components such as glutathione (GSH) to neutralize free radicals.The antioxidation effect of glutathione is determined by the 'probe' of this biochemical oscillator. Experiment indicates that it can be as a base of quantitative analysis. The method relies on the linear relationship between the changes in the amplitude of the biochemical oscillating and the concentration of GSH in the range 9.38×10 -7～7.50×10 -5 mol·L -1. Some possible mechanism of this reaction is discussed.

关 键 词：生物酶振荡器 谷胱甘肽 抗氧化效应 PO振荡反应 人 衰老 抗氧化剂 

分 类 号：O629[理学—有机化学]