类囊体膜光系统Ⅱ中超氧阴离子生成机制的研究  

Studies on Superoxide Formation in Photosystem Ⅱ with a Novel Spin Trap

作　　者：孙健[1] 刘科[1] 徐英凯[1] 陈中伟[1] 刘扬[1] 

机构地区：[1]中国科学院化学研究所分子科学中心分子动态和稳态结构国家重点实验室,北京100080

出　　处：《高等学校化学学报》2002年第5期979-981,共3页Chemical Journal of Chinese Universities

基　　金：国家重点基础规划项目(批准号:G1998010100); 国家自然科学基金(批准号:39890390;39870208)资助.

摘　　要：对高等植物类囊体膜的研究表明，易于损伤生物体的超氧阴离子自由基（O2- ）可以在其光系统Ⅱ（PSⅡ）中大量生成，并且可能是导致光系统的光抑制和光破坏现象的主要根源之一[1－3]．然而，由于超氧阴离子自由基化学性质活泼，而且生物体中含量很低，因此受物理检测手段的局限，对于PSⅡ中O2-的生成机制所知甚少．Abstract Under strong illumination, photosystem Ⅱ (PS Ⅱ )of higher plant was active in superoxide production. However, it keeps unclear about the mechanisms and sites of superoxide formation by PS Ⅱ . Concerning above questions, the molecular mechanisms of superoxide formation by PS II from spinach in this work has been studied. The site range for possible superoxide formation by PS I has been constricted by the study on the donor or acceptor inhibited PS I preparation. The results indicated that the plasto-quinones located at QA and QB of PS I was the primary site responsible for superoxide formation.

关 键 词：类囊体膜 光系统Ⅱ 氧阴离子自由基 生成机制 活性氧 自旋捕捉-ESR 植物 光合作用 

分 类 号：Q945.11[生物学—植物学]