圆二色谱和紫外可见光谱研究抗体-卟啉的相互作用  

Studied on the Interactions of Antibody-Porphyrin by Circular Dichroism and Ultraviolet-Visible Spectrophotometry

作　　者：祁超[1] 李伟国[1] 张玉静[2] 刘立岩[2] 赵大庆[1] 

机构地区：[1]中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春130022 [2]中国人民解放军军需大学生物化学教研室,长春130062

出　　处：《分析化学》2002年第5期601-604,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金 (2 970 10 0 5 );中国科学院基础局重大项目 (KJ95 1 A1 5 0 4 0 2 )资助课题

摘　　要：圆二色谱 (CD)和紫外可见光谱 (UV VIS)可用来研究单克隆抗体 (McAb) 卟啉之间的相互作用。卟啉形成McAb 卟啉复合物 ,在Soret带区域最大吸收峰有显著红移和增色现象。在 3 5 0～ 45 0nm区域 ,形成复合物时能检测诱导的CD光谱。CD光谱遵守朗伯 比尔定律 ,显示等吸收行为。McAb 卟啉复合物的紫外可见吸收及诱导的CD光谱在pH 6～ 1 1的范围内保持不变 ,说明复合物异常稳定 ,也说明McAb 卟啉结合位点之间疏水相互作用是主要因素。Circular dichoism and UV-vis measurements were used to study the interaction between porphyrin and monoclonal antibodies ( McAbs). McAbs-porphyrin complex formation is usually accompanied by significant bathochromic shift and hyperchromicity changes of the absorption maxima in the porphyrin soret band region. Induced CD spectra in the same region (350 similar to 450 nm) were detected upon complex formation. They follow Lamb-Beer's law and exhibit isosbestic behavior. Both the UV-Vis and induced CD spectra of the antibody: porphyrin complex remain unchanged over a broad pH range ( pH 6 similar to 11), indicating remarkable stability of the complex and reflecting the dominant role of hydrophobic interaction between the hapten benzophenone and the antibody combining site.

关 键 词：圆二色谱 紫外可见光谱 相互作用 单克隆抗体 meso-四(α α α-O-苯乙酰苯)卟啉 

分 类 号：O657.7[理学—分析化学] O657.3[理学—化学]