乌蛇散对肺纤维化模型作用的实验研究(Ⅱ)  

Effect of WuSheSan on the BleomycinA5-Induced Pulmonary Fibrosis in Rats

作　　者：宋建平[1] 潘梦菲[2] 李瑞琴[1] 李伟[1] 刘方州[3] 

机构地区：[1]河南中医学院,郑州450008 [2]上海市医药工业研究院 [3]河南省中医药研究院

出　　处：《中国中医药信息杂志》2002年第5期30-32,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：河南省科技攻关项目的部分内容(№.981170690)

摘　　要：目的:观察乌蛇散对肺纤维化的防治作用及机理。方法:大鼠气管滴入平阳霉素建立肺纤维化模型。用药组按原药材量3g/kg体重灌胃乌蛇散,每日1次,28d后处死,观察各组肺部病理损害程度,检测肺组织中去甲肾上腺素(NE)、多巴胺(DA)及5-羟色胺(5-HT)含量,并计数支气管肺泡灌洗液(BALF)中的炎症细胞总数及分类,进行组间比较。结果:模型组肺纤维化程度、肺组织中NE、DA、5-HT含量及BALF中的炎症细胞总数均显著高于正常对照组;乌蛇散组肺纤维化程度及肺组织中5-HT含量、BALF中炎症细胞总数均显著低于模型组。结论:乌蛇散能减轻平阳霉素所致大鼠肺纤维化程度,阻止炎症细胞及介质在肺内聚集可能是其作用机理。Objective:To observe the preventive effect and therapeutic mechanism of WuSheSan(WSS) in treating bleomycinA5-induced plmonary fibrosis of Rats. Methods: Pulmonary fibrosis was induced by bleomycinA5 in SD rats. In drug group WSS was given one time a day for 28 days, and then were killed. The lungs of all rats were harvested for histopa-thological examination. The contents of NE, DA and 5-HT in the lungs were measured and compared. Results: The degree of pulmonary fibrosis, the content of cell in BALF and NE、DA、5-HT in lung in the model rats taking WSS were significantly higher than that of the normal controlled rats. The degree of Pulmonary Fibrosis, the content of cell in BALF and 5-HT in lung in the rats taking WSS were significantly lower than that of the model rats. Conclusion: WSS could mitigate the degree of bleomycinA5-induced pulmonary fibrosis in Rats. It could be therapeutic mechanism to prevent the gathering of inflammatory cell and mediator from lung.

关 键 词：肺纤维化 乌蛇散 去甲肾上腺素 多巴胺 5-羟色胺 支气管肺泡灌洗液 药理 

分 类 号：R285.5[医药卫生—中药学]