非开胸法建立室壁瘤动物模型的实验研究  

A closed-chest pig model of aneurysm

作　　者：朱莉[1] 曹克将[1] 马根山[1] 杨志健[1] 王连生[1] 陈明龙[1] 邹建刚[1] 杨国平[1] 杨兵[1] 杨荣[1] 吴文霞[1] 徐云[1] 

机构地区：[1]南京医科大学第一附属医院心内科,210029

出　　处：《中国介入心脏病学杂志》2002年第2期103-106,共4页Chinese Journal of Interventional Cardiology

摘　　要：目的　探讨运用PTCA球囊封堵猪冠状动脉建立急性心肌梗死后室壁瘤的动物模型的实验方法。方法　选用家猪 7只 ,麻醉后经颈总动脉或股动脉置入PTCA球囊至左前降支 (LAD)第一对角支远端 ,对堵血流 15 0分钟。观察 :心电图、心肌酶、心脏二维超声检查及冠状动脉和左心室造影。结果　 7只猪均完成LAD的封堵 ,2只分别在堵闭 12 0分钟和 2 0分钟后因心室纤颤死亡。存活的 5只猪成功建立左室前壁急性心肌梗死模型 ,术后 6周造影复查示左室前壁、心尖部室壁瘤形成 ,4只猪堵闭的LAD远端闭塞。心电图显示急性心肌梗死的典型图形和动态演变过程。cTnI明显升高并呈动态演变。术后 1小时超声检查出现间隔上部及前壁局部运动异常 ,术后 2周即有室壁瘤形成。结论　运用PTCA球囊封堵冠状动脉可成功建立急性心肌梗死后室壁瘤的动物模型 ,与开胸法相比更接近人体的状态 ,具有创伤小、动物成活率高、生存时间长、技术要求不高等优点 ,可为进一步的研究提供较好的实验模型。Objective To explore and develop a close-chest animal model of acute myocardial infarction (AMI) with left ventricular aneurysm. Methods After anesthesia, 2.0 mm or 2.5 mm angioplasty balloon was positioned in the mid-LAD distal to the first diagnonal branch. The balloon was inflated and occluded the LAD for 150 minutes. ECG and blood pressure were monitored. UCG, cTnI and coronary angiography were also investigated to confirm AMI and the aneurysm. Results Five of seven pigs underwent successful induction of AMI and aneurysm. Two of seven pigs died of ventricular fibrillation. The dynamic changes of ECG and cTnI further confirmed AMI. Regional wall-motion abnormalities were found by two-dimensional echocardiography one hour after AMI and left ventricular aneurysm two weeks later in all pigs. Conclusion A closed-chest pig model of aneurysm after AMI is feasible and has a relatively high induction rate and a low mortality.

关 键 词：非开胸法 实验研究 动物模型 心肌梗死 PTCA球囊 

分 类 号：R542.22[医药卫生—心血管疾病]