体外培植干细胞复制肠粘膜组织的研究——在第十九届夏威夷国际烧伤会议上的报告  

Regeneration and Clone of Intestinal Organ by Culturing Stem Cells in vitro

作　　者：徐荣祥 王运平 范然 

机构地区：[1]美宝集团细胞组织工程研究室,北京100053

出　　处：《中国烧伤创疡杂志》2002年第2期65-71,共7页The Chinese Journal of Burns Wounds & Surface Ulcers

摘　　要：目的 :探索利用干细胞培植技术体外再生复制组织器官 ,并在体内验证。方法 :取孕 1 7天胚胎小鼠肠组织块在体外培养 ,实验分对照组和实验组 ,实验组培养液中加适量的促使肠粘膜复制的组合物GIC。连续观察、记录各组组织细胞生长情况。另在小鼠胃溃疡模型、胃和十二指肠溃疡病人观察GIC的疗效。结果 :胚胎鼠小肠组织块体外培养 ,对照组 1 0天后逐渐死亡 ;而实验组则持续生长、增殖、不断复制形成新的粘膜组织。GIC对动物和人消化道溃疡也有明显疗效 ,可使粘膜组织再生修复 ,无瘢痕愈合。结论 :体外复制肠粘膜组织器官是完全可以实现的 ,这不仅具有重要的组织修复医疗价值 ,而且为人体生命科学研究提供了从来没有过的肠粘膜组织活体动态模型。Objective: To explore the potential of regenerating and cloning tissues and organs in vitro by culturing stem cells, and to verify the results in vivo and in situ. Methods: Tissue explants of mouse small intestine was harvested from 17-day mouse embryos and cultured in vitro. The culture was divided into two groups: the control group cultured in normal tissue culture medium (complete MEM) and the experimental group in complete MEM with the addition of a tissue culture composition GIC that promoted the growth of intestinal mucosa. Continuously observe and record the growth of cells in two groups. In addition, the efficacy of GIC was also investigated by treating mice with gastric ulcers and patients with gastric and duodenal ulcers. Results: The culture results showed that cells in the control group never survived over 10 days in the culture, while cells in the experimental group continued to actively grow, proliferate and clone to form new mucosal tissue. GIC also showed obvious therapeutic effects on treating gastrointestinal ulcers both in animal model and patients by repairing and regenerating mucosa, which thereby resulted in scar-free healing. Conclusion: This result makes it absolutely possible for us to clone intestinal mucosa in vitro by using stem cells as the sources. It is not only valuable for tissue repair and regeneration in medical practice, but also providing a live, dynamic intestinal mucosa model system for life sciences research. This model system is the first one ever existed in the world.

关 键 词：干细胞 再生 器官复制 体外培养 肠 GIC 肠粘膜组织 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学]