下丘脑室旁核μ受体在大鼠烫伤休克中的作用  

The Role of Hypothalamic Paraventricular Opioid μ Receptor on Burn Shock in Rats

作　　者：洪新如 王成海[2] 林葆城[2] 朱鹤年[2] 

机构地区：[1]福州军区总医院儿科,福建福州350025 [2]第二军医大学基础部神经生物学教研室,上海200433

出　　处：《中国烧伤创疡杂志》2002年第2期78-81,共4页The Chinese Journal of Burns Wounds & Surface Ulcers

摘　　要：目的 :探讨下丘脑室旁核阿片 μ受体在介导大鼠烫伤休克中的作用。 方法 :2 1只大鼠随机均分为 3组 :DAGO组 (A)、β FNA组 (B)和单纯烫伤组 (C)。背部 2 0 %体表面积Ⅲ°烫伤后立即向下丘脑室旁核微量注射阿片μ受体激动剂DAGO或其拮抗剂 β FNA ,观察心功能指标和存活时间的变化。 结果 :①存活时间 :A、B、C组存活时间分别为 (480± 56)min、(545± 45)min和 (551± 2 6)min,A组显著短于B或C组 ;②平均动脉压 :A组烫后 90～ 450min时间上与烫前值的比值显著低于C组 ,B组烫后 1 50、51 0min显著高于C组 ,多数时间点上A组显著低于B组。③dP/dtmax:A组比值烫后 1 50、2 1 0、450min显著小于C组 ,B组比值烫后 0、2 70min显著大于C组 ,伤后 0～ 2 1 0min时间点上A组显著低于B组。④心率 :A组比值烫后 330、390和 450min显著小于B组 ,C组与A或B组之间差别无显著意义。结论 :下丘脑室旁核阿片 μ受体在介导大鼠烫伤后的病理生理改变中起重要作用 ,其激活可显著加快心功能指标的恶化 ,阻断其介导作用则具有对抗烫伤休克的作用 。Objective:To investigate the role of hypothalamic paraventricular opioid μ receptor on burn shock in rats.Methods:Male SD rats were randomized into DAGO group(A)?β FNA group(B) and merely scald wounded group(C).DAGO(A)or β FNA(B)both at 100 ng/2 μ1 was administered intraparaventricularly immediately after a third degree burn over about 20% of the total body surface area resulted from the shaved back of the rat being dipped into 100℃ water for 20 s.Cardiovascular parameters(mean arterial pressure,dP/dt max and heart rate)were recorded different time intervals following the microinjection.Survival times were also recorded.Results:①Survival times of A,B and C groups were(480±56)min,(545±45)min and (551±26)min,respectively with animals of group A lived statistically shorter than those of either group B or C.②Magnitude of decrease of ratio of mean arterial pressure in group A was obviously greater than that in group C during 90～450 min time points observed.This was also greater in group B vs C at 150 and 510 min. At most time points we observed,ratios of group A were statistically lowered than those of group B.③Values of ratio of dP/dt max were significantly smaller in group A vs group C at 150,210and 450min,and the valuse showed markedly greater in group B than in group C at 0 and 270 min.Ratios of group A were statistically lowered than those of group B at the time points of during 0～210min period.④Ratios of heart rate of group A was smaller t·han those of group B at 330,390 and 450min.There were no significant differeces between group C and either group A or group B all the time points.Conclusions:Present data suggest that hypothalamic paraventricular opioid μ receptor plays a key role in process of pathophysiological changes after burn shock in rats.Cardiovascular parameters are significantly deteriorated as μ receptor was activated either by its exogenous agonist or by endogenous mechanism resulted from the jnjury.Blockade of this activation and function of μ receptor is

关 键 词：Μ受体 下丘脑室旁核 烫伤 大鼠 SD 

分 类 号：R644[医药卫生—外科学]