反相高效液相色谱法测定牙周康胶囊中甲硝唑和芬布芬的含量  

Determination of metronidazole and fenbufen in compound capsule by RP-HPLC

作　　者：王铁杰[1] 罗贞贞[2] 颜晗[1] 

机构地区：[1]深圳市药品检验所,广东深圳518029 [2]广东药学院,实习生广东广州510224

出　　处：《中国医院药学杂志》2002年第5期285-286,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立同时测定牙周康胶囊中甲硝唑和芬布芬的方法。方法 :采用反相高效液相色谱法 ,以Lichrospher ODS(5μm ,4 .0mm× 2 5 0mm)为色谱柱 ,甲醇 水 (75∶2 5 )为流动相 ,检测波长 2 85nm ,峰面积外标法定量。结果 :平均回收率 :甲硝唑为 99.5 % ,RSD 0 .6 % (n =5 ) ;芬布芬为 10 0 .0 % ,RSD 0 .7% (n =5 )。结论 :方法简便 ,快速 ,准确可靠 ,适于该制剂的含量测定。OBJECTIVE To establish a method for the determitation of both Metronidazole and Fenbufen in compound capsule.METHODS RP HPLC was adopted with Lichrospher ODS(5 μm, 4.0 mm×250 mm) as analytical column, methanol water(75∶25) as the mobile phase, detection wavelength of 285 nm and external standard method.RESULTS The average recovery: Metronidazole was 99.5% ,RSD 0.6% ( n =5); Fenbufen was 100.0% ,RSD 0.7% ( n =5).CONCLUSIONS The method is simple, rapid, reliable for assay of the preparation.

关 键 词：反相高效液相色谱法 牙周康胶囊 甲硝唑 芬布芬 含量测定 

分 类 号：R988.2[医药卫生—药品] R927.2[医药卫生—药学]