HFRS病毒滇绒鼠分离株E-142全S基因序列分析  

Sequences Analysis of Complete S gene of Hantavirus E-142 Strain from Yunnan E.eleusis

作　　者：姚智慧[1] 董关木[1] 俞永新[1] 自登云 张海林 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]云南省流行病学研究所

出　　处：《中国公共卫生》2002年第5期529-530,共2页Chinese Journal of Public Health

摘　　要：目的　了解肾综合征出血热病毒云南地区滇绒鼠分离株E - 14 2的分子生物学特征。方法　应用PCR方法克隆了该株全S基因。结果　序列测定结果表明E - 14 2株的S片段的全基因序列共 170 2个核苷酸 ,编码 4 2 9个氨基酸。四种核苷酸的比例分别为A32 79% ,G2 5 12 % ,T2 3 0 2 % ,C19 0 7% ,GC含量为 4 4 19% ,AT含量为5 5 81%。E - 14 2与汉坦病毒S基因核苷酸和氨基酸同源性比较表明 ,E - 14 2株与HTN型同源率为 86 6 %～90 4 % ,而与SEO型仅为 6 8 3%～ 6 9 6 % ,E - 14 2株病毒属于HTN型 ,但与HTN型其它毒株相差高达 9 6 %～13 4 %。结论　E - 14 2株病毒是HTN型的新亚型毒株。Objective For understanding the molecular characteristics of Hantavirus E-142 strain isolated from Yunnan E.Eleusis.Methods The S complete gene was amplified by RT-PCR and the purified products was cloned into T vector.Results Complete S segment of E-142 was 1 702 nucleotides in length encoding 429 amino acids.It showed 86 6%-90 4% homology with HTN virus,but only 68 3%-69 6% homolgy with SEO viruses at nucleotide level.Conclusion The results suggested that the E-142 strain was a new subtype of HTN virus.

关 键 词：HFRS病毒 基因序列 肾综合征出血热 E-142株 S基因 动物实验 

分 类 号：R512.803[医药卫生—内科学]