幽门螺杆菌的分离与特性研究  

Study on Isolation,Characteristics and Comparison of Several Detection Methods of Helicobacter Pylori

作　　者：丁云菲[1] 李安明[2] 李德华 陈德高 戴长柏[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所分子生物室,昆明650118 [2]成都地奥制药集团有限公司新药部 [3]中国科学院成都生物研究所

出　　处：《中国公共卫生》2002年第5期545-547,共3页Chinese Journal of Public Health

基　　金：中国科学院成都地奥科学基金 (DASF)资助

摘　　要：目的　分离一株幽门螺杆菌 ,研究它的生物学特性及免疫原性 ,同时比较几种检测手段的优势。方法　采用厌氧微生物培养技术及PCR检测方法分离、初步鉴定幽门螺杆菌。重点对其中一株菌HP -WGM做各项生理生化实验 ,ELISA试验分析。结果　从 10 6位患者中分离鉴定得到 4 0株疑似幽门螺杆菌。HP -WGM菌株革兰氏染色阴性、杆状、稍弯曲、菌体的一端着生数根带鞘鞭毛。该菌脲酶、触酶、氧化酶均为阳性 ,不还原硝酸盐 ,H2 S的生成试验阴性 ,其生长耐受 3 5 %NaCl、l%甘氨酸 ,水解淀粉 ,不液化明胶。该菌有一定的免疫原性。结论　HP -WGM菌株可作为一种抗原用于检测幽门螺杆菌的感染程度。对于疾病的确诊 ,应采用至少 2种以上的检测手段 ;用于科学研究时 ,细菌培养、鉴定的方法才是最为可靠的手段。Objective To study the isolation,characteristics and comparison of several detection methods of Helicobacter pylori.Methods Anaerobic cultivation and PCR were used to isolate and determine H.Pylori preliminarily.One strain was further determined by various tests of characteristics.Results About forty strains of H.pylori were isolated and preliminarily identified from gastric mucosa biopsy specimens in 106 patients presenting for gastroscopy at hsopitals.HP-WGM was gram-negative,curved bacilli which ha smooth coats,several sheathed single polar flagella.After 2～3 days'incubation,it grew evidently as about 1mm in diameter,round,protruding,uniform in edge,semi-translucent coloies on urea agar rolled-tubes incubated at 37℃ under microaerophilic condition.It was positive in urease,catalase,oxydase tests and negative in H 2S producing and gelatin liquefaction tests.It couldn't reduce NO - 3,could tolerate 3 5% NaCl and 1% glycine and hydrolyze starch.The organism is immunogenic.Conclusion HP-WGM could be used as an antigen to carry out ELISA for determining the extent of H.pylori infection.At least over two methods are necessary to diagnose the infection.But in scientific research,the culture and determination of bacteria is the most reliable way.

关 键 词：幽门螺杆菌 检测 细菌培养 鉴定 

分 类 号：R378.2[医药卫生—病原生物学]