生后发育期铅接触对大鼠海马组织的影响  

Effect of Lead on Hippocampi Tissues of Mature Rats During Time of Growth After Birth

作　　者：李鹏[1] 张雪梅[2] 丁颖[3] 

机构地区：[1]沈阳市疾病预防控制中心,110031 [2]沈阳市预防医学研究所 [3]沈阳医学院第二附属医院

出　　处：《中国公共卫生》2002年第5期566-568,共3页Chinese Journal of Public Health

摘　　要：目的　观察铅对生后发育期大鼠海马神经生长因子 (NGF)基因表达的影响。方法　仔鼠出生后 ,即给母鼠饲以 0 2 %醋酸铅 ,仔鼠通过母乳摄入铅。采用RT -PCR方法 ,半定量分析生后发育期铅接触仔鼠海马组织NGFmR NA含量的变化。结果　与对照组相比 ,铅染毒仔鼠血铅和海马组织铅含量明显升高 (P均 <0 0 0 1) ,而海马组织NGFmRNA含量却显著下降 (P <0 0 0 1)。结论　铅染毒可使生后发育期仔鼠海马组织NGFmRNA表达量下降。Objective To observe the effect of lead on the expressions of gene of NGF of hippocampi tissues of mature rats during the time of growth after birth.Methods To have the mother rat assimilate 0.2% of lead acetate after the birth of the young,the young rat assimilate lead through the milk.And adopt the method of RT-PCR to semi-quanititatively analyse the change of the content of NGF mRNA in the hippocampi tissues of the young rat after the contact with lead during time of growth after birth.Results Compared with the control group.The content of lead in the lead-polluted young rat the hippocampi tissue went up,while the content of NGF mRNA in the hippocampi tissue went down.Conclusion The pollution of lead may reduce the quantity of the show of NGF mRNA in the hippocampi tissues of young rat when they were in the time of growth after the birth.

关 键 词：铅 NGFMRNA 海马 生后发育 动物实验 

分 类 号：R995[医药卫生—毒理学] R338.26[医药卫生—药学]