壳聚糖中药复合药膜治疗大鼠体表溃疡的机理研究  

Basic Mechanism of Compound Chitosan Herbal Membrane in Treatment of Skin Ulcer

作　　者：李令根[1] 赵钢[1] 吴明远[1] 王春明 蔡伟民[3] 吴迪[3] 张毅奎[1] 李为 

机构地区：[1]黑龙江中医药大学附属第一医院,哈尔滨150040 [2]哈尔滨市中医院 [3]哈尔滨工业大学

出　　处：《中国中西医结合外科杂志》2002年第2期80-83,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：黑龙江省自然科学基金资助 (No .D990 5 )

摘　　要：目的 :研究壳聚糖中药复合药膜治疗体表溃疡的机理。 方法 :建立大鼠背部溃疡模型后 ,将动物分为 3组 ,分别外敷壳聚糖中药复合药膜、生肌玉红膏、凡士林。并于用药后 4、7、11d取疮面组织 ,比较各组动物溃疡坏死层厚度、炎性渗出层厚度、炎细胞浸润量、肉芽厚度、新生毛细血管量、纤维增生及毛细血管内皮生长因子 (VEGF)、增殖细胞核抗原 (PCNA)、吞噬细胞 (Mδ)的情况。 结果 :壳聚糖中药复合药膜组的肉芽厚度增加 ,水肿渗出较少 ,新生毛细血管较多 ,大多直立生长且充血明显 ,Mδ、VEGF、PCNA呈逐渐增多趋势 ,与其它两组相比有显著差异。 结论 :壳聚糖中药复合药膜通过刺激机体分泌大量Mδ 及促进VEGF、PCNA释放 ,促进肉芽组织生长 ,减少纤维组织的增生 。Objective:To investigate the basic mechanism of Compound Chitosan herbal membrane in the treament of skin ulcer Methods:Back ulcer models were established in rats,then the rats were divided into three groups and treated with compound Chitosan herbal membrane,vaseline gouze and Shengjiyuhong plaster respectively. Tissue biopsy specimens of the ulcer surfaces were separately taken after 4days,7days,and 11days, external application of medication the thickness of the necrotic layer,inflammatory exudative layer,infiltration phlogotic cell number,thickness of granulation, amount of regenerative capillary vessels were determined pathologically,and vascular endothelium growth factor(VEGF),proliferative cellular nucleolus antigen(PCNA)and phagocyte M δ dtermined by immunohistochemistry.Results:The thickness of granulation increased with less edema and exudation in compound Chitosan herbal membrane group,highly vascular growing with obvious hyperemia,M δ,VEGF and PCNA increased markedly with significant differences.Conclution:compound Chitosan herbal membrane can cure the skin ulcer by stimulating the organism to secrete lost of M δ,VEGF and PCNA,promoting the growth of granulation and reducing the hyperplasia of fibrous tissue.

关 键 词：壳聚糖 复合药膜 皮肤溃疡 中药 动物实验 

分 类 号：R285.5[医药卫生—中药学]