中国O139群霍乱弧菌核糖体基因多态性的研究  

A study on genetic polymorphism of rRNA gene pattern of Vibrio cholerae O139 in China

作　　者：曲梅[1] 阚飙[1] 祁国明[1] 刘延清[1] 高守一[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所卫生部医学分子细菌学重点实验室

出　　处：《中华流行病学杂志》2002年第3期203-205,共3页Chinese Journal of Epidemiology

基　　金：国家自然科学基金资助项目 (3 9870 0 3 1)

摘　　要：目的　分析国内O139群霍乱弧菌分离株的遗传多态性。方法　以 16srRNA和 2 3srRNA核糖体基因作探针 ,对内切酶消化的菌株染色体DNA进行Bouthern杂交 ,分析杂交图谱。结果　选择的 12 2株O139群霍乱弧菌得到 10种核糖体基因型。 9株ctxAB、zot和RS均为阴性的无毒菌株 ,分属于 4种独特的核糖体基因型。结论　O139群霍乱弧菌在遗传分化及克隆群的地区分布上存在多态性 ,提示国内O139霍乱流行的复杂性。Objective To investigated the genetic polymorphism of the isolated strains using ribotyping method. Methods One handred twenty two strains of V. cholerae O139 isolated from different areas of China from 1993 to 1999 were selected and characterized with ribotyping, including 16s rDNA and 23s rDNA probes. Results One handred twenty two strains were differentiated into 10 different ribotypes (RT1 RT10) on the basis of rRNA gene probes hybridization (with BglⅠ digestion), which consisted of 7 9 bands between 12 and 1.5 kb in size. RT1 and RT3, as two predominant ribotypes, comprised most number of the strains which spread to the extensive range. Nine strains, which are negative to ctxAB, zot and RS individually, belong to 4 special ribotypes. The dendrogram revealing genetic relationship among different clones of V. cholerae O139 showed that the clones belonging to RT1 and RT2 had genetic similarity on high degree, although they were isolated from different regions. The two predominant ribotypes (RT1 and RT3) were distant in genetic relationship. Conclusion Results showed the clonal diversity and the wide area distribution of V. cholerae O139 strains in China, suggesting the multiple origins of O139 epidemics.

关 键 词：O139群霍乱弧菌 核糖体基因分型 中国 遗传多态性 

分 类 号：R378[医药卫生—病原生物学]