成人骨髓间质干细胞基本生物学特性  

Basic Biological Characterics of Human Bone Marrow Derived Mesenchymal Stem Cells

作　　者：张丽蓉[1] 陈振光[2] 项鹏[1] 夏文杰[1] 张秀明[1] 李艳[1] 李树浓[1] 

机构地区：[1]中山大学中山医学院病理生理教研室,广东广州510089 [2]中山大学附属第一医院心胸外科,广东广州510089

出　　处：《中山医科大学学报》2002年第3期170-172,F004,共4页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：国家自然科学基金资助项目 (3 0 10 0 188) ;广东省"十五"重大专项基金资助项目 (A3 0 2 0 10 1)

摘　　要：【目的】研究成人骨髓间质干细胞 (MSC)基本生物学特性。【方法】采用Ficoll Paque淋巴细胞分离液分离成人MSC ,体外扩增 ,比较不同培养基和血清含量对MSC生长的影响 ,流式细胞仪检测MSC表面抗原表达。【结果】成人骨髓间质干细胞在体外扩增原代可获得 (5～ 6 )× 10 5个细胞 ,15代可获得 (3～ 4 )× 10 12 个细胞 ,随着传代次数的增加 ,细胞的增殖能力下降。L DMEM培养基有利于细胞的培养扩增。细胞流式细胞仪检测结果显示CD2 9、CD4 4、CD5 9、CD10 5、CD16 6表达阳性 ,CD11a、CD14、CD33、CD34、CD4 5、CD38、CD80、CD86、CD117表达为阴性。【结论】MSC有很强的自我更新能力 。To investigate the basic biological characteristics of human mesenchymal stem cells. hMSC were separated from human marrow with Ficoll Paque reagent and expanded in culture medium The proliferation and growth characteristics were observed in primary and passage culture To detect the surface antigens, the labeled cells were analysed on a FACScan flow cytometer The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast like morphology hMSC have a strong self renewal capacity After primary culture approximately (5～6)×10 5 cells were obtained After passage 15, it to be got (3～4)×10 12 cells The proliferation ability was decreased gradually after several passages The decline of CFU Fs with passage numbers was apparent from assays of three samples from different donors and some spindle shaped cells acquired a broad morphology and reduced replication capacity L DMEM was better for proliferation and growth of MSC These expanded attached MSC were uniformly positive for CD29、CD44、CD59、CD105、CD166 and didn't express CD11a、CD14、CD33、CD34、CD45、CD38、CD80、CD86、CD117 [Conclusion] hMSCs have strong self renewal capacity and can be used as the seed cells for tissue engineering

关 键 词：间质干细胞 骨髓 细胞增殖 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]