克拉霉素对铜绿假单胞菌生物被膜的影响  

Influnence of clarithromycin on biofilm of Pseudomonas aeruginosa

作　　者：王睿[1] 裴斐[1] 柴栋[1] 朱曼[1] 方翼[1] 王中孝[1] 刘皈阳[1] 

机构地区：[1]解放军总医院临床药理研究室,北京100853

出　　处：《中国抗生素杂志》2002年第5期293-297,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金资助课题 (批准号 30 0 70 72 0 )

摘　　要：目的 :研究克拉霉素对铜绿假单胞菌生物被膜生物特性的影响。方法 :采用结晶紫染色法测定生物被膜中细菌的粘附性 ;浓度梯度渗透法测定生物被膜的渗透性 :采用卡唑 /乙醇法测定克拉霉素对藻酸盐的合成抑制作用。结果 :采用平板法 7d可建立稳定的生物被膜 ,1/4MIC克拉霉素与 8×MIC加替沙星联合应用后光密度值由 ( 0 .12 6± 0 .0 11)显著的降低至 ( 0 .114± 0 .0 12 ) ,4μg/ml组、8μg/ml组、16 μg/ml组加替沙星的渗透浓度 ( μg/ml)可由 ( 1.2 10± 0 .0 91)、( 2 .911± 0 .112 )、( 5 .911± 0 .2 13)显著的增加至 ( 1.730± 0 .132 )、( 3.911±0 .111)、( 7.90 2± 0 .35 4) ;80 μg/ml、40 μg/ml、2 0 μg/ml、10 μg/ml克拉霉素组生物被膜中藻酸盐的含量 ( μg/108CFU)由 ( 10 .0 7± 0 .5 5 )显著的降低至 ( 2 .34± 0 .2 1)、( 4 .91± 0 .16 )、( 7.2 2± 0 .36 )、( 8.82± 0 .5 0 )。结论 :克拉霉素可降低铜绿假单胞菌生物被膜中细菌的粘附性 ,增强加替沙星在生物被膜中的渗透能力 ,抑制铜绿假单胞菌藻酸盐的合成。Objective: To assess the influence of clarithromycin on Pseudomonas aeruginosa biofilm. Methods: To determine the adherence of mucoid Pseudomonas aeruginosa by crystal violet stained method; the gatifloxacin penetration concentration was determined by HPLC and the effects of clarithromycin on Pseudomonas aeruginosa biofilm was determined by cazole/ethanol method. Result: The stable biofilm could be formed in 7 day. When 8×MIC gatifloxacin was combined with 1/4MIC clarithromycin, the adherence of mucoid Pseudomonas aeruginosa decreased significantly, the optical density decreased from (0.126±0.011) to (0.114 ±0.010). The penetrated concentrations of gatifloxacin through biofilm were increased from (1.210±0.091), (2.911±0.112) and (5.911±0.213) to (1.752±0.122), (3.908±0.154) and ( 7.898 ±0.321) μg/ml in three dosages 4.8 and 16μg/ml respectively, and the concentration of alginate in biofilm was decreased from (10.07±0.55) to (2.34±0.21), (4.91±0.16), (7.22±0.36), (8.82±0.50)μg/10 8 CFU in 80, 40, 20, 10μg/ml clarithrnycin group respectively. Conclusion: Clarithromycin could reduce the adherence of Pseudomonas aeruginosa while enhance the penetration ability of gatifloxacin and inhibit the alginate synthesis of Pseudomonas aeruginosa .

关 键 词：生物被膜 铜绿假单胞菌 克拉霉素 加替沙星 

分 类 号：R965[医药卫生—药理学]