Fas/FasL途径在柔红霉素诱导HL-60及U937细胞凋亡中作用的研究  

The effect of the Fas/FasL pathway during daunorubicin induced apoptosis of HL-60 and U937

作　　者：刘芳[1] 向建平[1] 

机构地区：[1]华中科技大学同济医学院血液病研究所,武汉430022

出　　处：《临床血液学杂志》2002年第3期118-119,共2页Journal of Clinical Hematology

摘　　要：目的 :研究柔红霉素 (DNR)诱导白血病细胞凋亡的机制 ,进一步探讨Fas/FasL途径在DNR诱导白血病细胞凋亡中的作用 ,并评价sFasL与DNR联用的致凋亡效应。方法 :将DNR作用于白血病细胞系HL 6 0、K5 6 2、U937细胞 ,并用流式细胞术 (FCM)检测其Fas抗原表达的改变。sFasL、DNR诱导白血病细胞凋亡 ,以抗Fas单抗 (IgG1)阻断Fas/FasL途径 ,借助原位末端标记法 (TUNEL)和FCM检测白血病细胞凋亡率。结果 :DNR处理HL 6 0、K5 6 2、U937细胞 18h后 ,Fas表达阳性率无明显变化 (P >0 .0 5 ) ,与sFasL联合作用后显示出协同效应。IgG1不能阻断DNR致白血病细胞凋亡的作用 ,但可阻断sFasL致白血病细胞凋亡的作用。结论 :DNR致白血病细胞株U937、HL 6 0凋亡的作用不依赖于Fas/FasL途径 ,但sFasL与DNR联合应用可协同增强抗肿瘤效应。Objective:The mechanism of daunorubicin (DNR) induced apoptosis in leukemic cells was studied to further investigate whether Fas/FasL system was involved in apoptosis induced by DNR and assess their effects. Method:The expression of Fas on human leukemic cell lines K562, HL 60 and U937 treated with DNR was detected by using fIow cytometry. The activities of sFasL. DNR inducing apoptosis of leukemic cells, in the absence or presence of neutralizing anti Fas IgG antibody, were detected by using flow cytometry and TUNEL.Result: Flow cytometric profiles of K562, HL 60 and U937 cells treated with DNR failed to show any significant increase in Fas expression over l8 h (P>0.05). sFasL has synergistic effect when used in combination with DNR. Conclusion:DNR induced apoptosis in human leukemic cells (U937. HL 60) is independent of the Fas/FasL system. But combination of sFasL and drug treatment produces a synergistic cytotoxic effect.

关 键 词：抗肿瘤药 柔红霉素 凋亡 白血病细胞 FAS配体 

分 类 号：R979.1[医药卫生—药品]