急性放射性皮肤溃疡形成早期应用生长因子的意义  

The changes and significance for b-FGF and VEGF expression in acute radia tion-induced skin ulcers

作　　者：曹卫红[1] 杨志祥[1] 谷庆阳[2] 王德文[2] 高亚兵[2] 杨文峰[1] 孙向黎[1] 

机构地区：[1]军事医学科学院附属医院,北京100039 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《中国临床康复》2002年第10期1422-1423,W002,共3页Chinese Journal of Clinical Rehabilitation

基　　金：基金项目:国家自然科学基金资助项目(39470229);"九五"军队医药卫生科研基金课题(96Q036)

摘　　要：目的探讨急性放射性皮肤溃疡形成早期b-FGF、VEGF及其受体的表达和意义。方法以50Gy60Coγ射线单次局部照射建立急性放射性皮肤溃疡动物模型,以手术方法建立单纯皮肤伤口动物模型,采用免疫组化、原位杂交等方法检测伤口及溃疡组织内b-FGF、VEGF及其受体的表达。结果创伤组伤后5～10d,肉芽组织内b-FGF、VEGF表达呈强阳性;照射后21～28d,放射性溃疡肉芽组织内b-FGF、VEGF表达呈阳性,但较创伤后5～10d明显减弱。结论辐射诱导的b-FGF、VEGF表达减弱与急性放射性皮肤溃疡愈合延迟有关。Objective To study the changes and significance for b-FGF and VEGF expression in early stage of acute radiation-induced skin ulcers.Methods A model of normal skin wounds was made.Another model was made with 50Gy 60 Co γ-rays .Immunohistochemical staining,in situ hybridization(ISH)were used to detect the expression of b-FGF and VEGF in simple skin wounds a nd acute radiation-in-duced skin ulcers.Results In simple skin wounds group,the expr ession of b-FGF and VEGF was strongly positive in granulation tissues on 5to 10days after injury;while in radi ation group,the expression of b-FGF and VEGF was weaker in granulation ti ssues on 21to 28days after radiation,compared with simple ski n wounds group on 5to 10days after inj ury.Conclusion Radiation-induced reduction of b-F GF and VEGF was relevant to the wound healin g retardation of acute radiation-in duced skin ulcers.

关 键 词：急性放射性皮肤溃疡 创面愈合 生长因子 

分 类 号：R818.895[医药卫生—放射医学]