T淋巴瘤TCR β链独特型DNA疫苗在小鼠骨骼肌中的基因及蛋白表达  

Gene and protein expression of idiotype DNA vaccine of TCRβ chain of T cell lymphoma

作　　者：张明智[1] 李继昌[2] 姜祖光 陈长英 乐晓萍[5] 

机构地区：[1]郑州大学第一附属医院肿瘤科,郑州450052 [2]郑州大学第一附属医院消化内科,郑州450052 [3]中原油田总医院肿瘤科,濮阳457000 [4]郑州大学基础医学院组织胚胎学教研室,郑州450052 [5]郑州大学第一附属医院护理部,郑州450052

出　　处：《郑州大学学报（医学版）》2002年第3期280-283,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：博士基金资助课题

摘　　要：目的:检测T淋巴瘤TCR β链独特型DNA疫苗的基因表达。方法:大剂量提取质粒,将BALB/c鼠随机分为 pcDNA3．l组、pcDNA3．1/TCR Vβ8组、pcDNA3．1/TCR Vβ8＋IL－2组、pcDNA3．l/TCR Vβ8+CpG+脂质体组和全硫代CpG组共5组(每组6只),双侧股四头肌内注射疫苗免疫,于第0、2、4周各免疫1次,共3次。接种疫苗后 5 d,用 RT-PCR法检测重组质粒mRNA表达。接种疫苗后 7 d应用免疫组化检测重组质粒蛋白表达。结果:pcDNA3．1/TCR Vβ8组、pcDNA3．l/TCR Vβ8＋IL－2组、pcDNA3．l/TCR Vβ8+CpG＋脂质体组小鼠骨骼肌中均显示mRNA和蛋白表达,而pcDNA3．1组和全硫代CpG组未见mRNA和蛋白表达。结论:T淋巴瘤TCRβ链独特型DNA疫苗在小鼠骨骼肌中有效地表达了基因产物。Aim: To test the gene expression of idiotype DNA vaccine of TCR β chain of T lymphoma . Methods: A large quantity of plasmids were extracted . The BALB/c rats were randomly allocated into 5 groups (six rats in every group) : pcDNA3. 1, pcDNA3. 1 /TCR Vβ8, pcDNA3.1/ TCR Vβ8 + IL-2, pcDNA3.1/ TCR Vβ8 + CpG+ liposome and thiosulfate-CpG. Vaccines were injected in bilateral quadriceps femorises once at the 0, 2nd and 4th week, respectively. The mRNA expression of recombinant plamids were tested with RT-PCR method on the 5th d after immunization and the protein expression of recombinant plasmid was tested with immunohistochemistry method on the 7th d after immunization. Results: It shows that there were the mRNA and protein expressions in skeletal muscles of the rats in the pcDNA3. 1 /TCR Vβ8 group, pcDNA3.1/ TCR Vβ8 + IL-2 group, and pcDNA3. 1/ TCR Vβ8 + CpG+liposome group while no mRNA or protein expression in skeletal muscles of the rats in the pcDNA3. 1 group and thiosulfate-CpG group. Conclusion: The idiotype DNA vaccine of TCR β chain of T lymphoma can effectively express gene production in the skeletal muscles of rats.

关 键 词：T淋巴瘤 TCR 独特型 重组质粒 DNA疫苗 基因表达 动物实验 

分 类 号：R318.5[医药卫生—生物医学工程] R733.4[医药卫生—基础医学]