骨髓基质细胞对神经干细胞分化为神经元的影响  

Effect of bone marrow stromal cells on the differentiation of neural stem cells into neurons

作　　者：娄淑杰[1] 顾平[1] 李怡[1] 陈菲[1] 张明徽[2] 何成[1] 王铭维[3] 路长林[1] 

机构地区：[1]第二军医大学神经生物学教研室,上海200433 [2]第二军医大学免疫学教研室,上海200433 [3]河北医科大学第五医院神经内科,河北石家庄050013

出　　处：《中国神经科学杂志》2002年第2期490-494,共5页

基　　金：国家自然科学基金资助项目 (3 0 170 469)

摘　　要：采用细胞共培养方式和免疫化学染色方法 ,研究骨髓基质细胞对神经干细胞分化为神经元、星形胶质细胞和寡突胶质细胞的影响。实验发现 ,体外培养的中脑神经干细胞在与成年大鼠骨髓基质细胞共培养 7d后 ,在神经干细胞后代中神经元比例可达 38.6 %± 10 .8% ,明显高于自然分化组 2 0 % ,提示骨髓基质细胞提供的微环境可明显提高神经干细胞后代中神经元的比例。The influence of bone marrow stromal cells on differentiation into neurons, astrocytes and oligodendrocytes of neural stem cells was investigated by directly plating neurosphere onto bone marrow stromal cells. The number of neurons, astrocytes and oligodendrocytes was obtained by using immunocytochemistry technique. The results show that the proportion of neurons was significantly increased by 2 fold in the co culture neural stem cells with bone marrow stromal cells for 7 d compared cultures of neural stem cells alone,we conclude that environmental cues provided by bone marrow stromal cells could increase the proportion of neurons in progeny of neural stem cells.\[

关 键 词：神经干细胞 骨髓基质细胞 神经元 细胞分化 细胞共培养方式 免疫化学染色 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]