HPLC法和FPIA法测定人血清中艾司唑仑浓度及其临床意义  

Estazolam in Human Serum Concentration Determined by HPLC and FPIA and Their Clinical Significance

作　　者：余爱荣[1] 吴笑春[1] 冷金华[1] 蒋兆健[1] 

机构地区：[1]广州军区武汉总医院临床药理科,430070

出　　处：《中国药师》2002年第6期330-331,共2页China Pharmacist

摘　　要：目的:比较高效液相色谱法(HPLC)和荧光偏振免疫法(FPIA)测定艾司唑仑中毒患者的血药浓度的结果,为临床准确测定其血药浓度提供参考。方法:采用HPLC法和FPIA法分别测定了71份艾司唑仑中毒患者的血标本,并对两种方法的测定结果作了统计学分析。结果:两种方法的测定结果无显著性差异(P>0.05),相关性较好,回归方程为Y(FPIA测定值)=1.127X(HPLC测定值)+0.056 7,r=0.989。结论:HPLC法和FPIA法均能用于监测艾司唑仑中毒患者的血药浓度,能较准确地反映中毒原因及中毒的严重程度,为临床制定抢救方案提供客观依据。Objective: To compare both serum concentration result of the accurate degree of the estazolam determined by HPLC and FPLA to provide a accurate serum concentration for clinic . Methods: The estazolam serum concentrations in 71 blood samples were determined by HPLC and FPIA. Results: There was no significant difference by two determining methods (P>0.05).The linear regression equation was Y = 1.127 X + 0.056 7( r = 0.989). Conclusion:The two determining methods are feasible for the clinical monitoring of estazolam concentration in human . They can also accurately reflect the reason of intoxication and the severity of intoxication, and to be served as an objective evidence of clinical rescue regimen.

关 键 词：血清 HPLC FPIA 艾司唑仑 血药浓度 液相色谱法 荧光偏振免疫法 安定类药物 

分 类 号：R927.2[医药卫生—药学] R971.3